Isolation by PCR of a cDNA clone from pea petals with similarity to petunia and wheat zinc finger proteins

Anthony J. Michael, Julie M I Hofer, T. H. Noel Ellis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The C2H2 TFIIIA/Krüppel class of zinc finger proteins are an important group of regulatory nucleic acid binding factors and have been extensively studied in humans, Drosophila and yeast. We have employed 3' RACE PCR, using a highly degenerate oligonucleotide primer, for the facile isolation of a C2H2 zinc finger protein cDNA (Pszfl) from pea petals. The Pszfl cDNA open reading frame potentially encodes a protein with two widely separated zinc fingers similar to zinc finger proteins from petunia and wheat. This class of two-fingered zinc finger proteins, possessing a wide and variable linker sequence, appears to be unique to plants. Three regions outside the zinc finger domains are also conserved between the members of the plant zinc finger protein family and one of these regions is a candidate nuclear localisation signal. The Pszfl amino acid sequence is most similar to that of the petunia Epfl protein, they possess an interfinger linker sequence of approximately the same length and they have a similar expression pattern with maximal transcript accumulation in mature petals, suggesting that Pszfl may be the pea homologue of the petunia Epfl zinc finger gene.

Original languageEnglish (US)
Pages (from-to)1051-1058
Number of pages8
JournalPlant Molecular Biology
Volume30
Issue number5
StatePublished - 1996

Fingerprint

Petunia
zinc finger motif
Zinc Fingers
Peas
corolla
Triticum
Zinc
peas
Complementary DNA
Clone Cells
clones
Polymerase Chain Reaction
wheat
Proteins
proteins
Transcription Factor TFIIIA
Nuclear Localization Signals
DNA Primers
nuclear localization signals
DNA primers

Keywords

  • Degenerate oligonucleotide
  • Pea petal
  • RACE PCR
  • Zinc finger

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Isolation by PCR of a cDNA clone from pea petals with similarity to petunia and wheat zinc finger proteins. / Michael, Anthony J.; Hofer, Julie M I; Noel Ellis, T. H.

In: Plant Molecular Biology, Vol. 30, No. 5, 1996, p. 1051-1058.

Research output: Contribution to journalArticle

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