Isolation of a recombinant copy of the gene encoding C/EBP.

W. H. Landschulz, P. F. Johnson, E. Y. Adashi, B. J. Graves, S. L. McKnight

Research output: Contribution to journalArticle

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Abstract

In two previous studies we described the properties of a heat-stable DNA-binding protein present in rat liver nuclei. This protein, hereafter termed C/EBP, is capable of selective binding to the CCAAT homology of several viral promoters (Graves et al. 1986), as well as the core homology common to many viral enhancers (Johnson et al. 1987). We now report the isolation of a recombinant clone of the gene that encodes C/EBP. Expression of the clone in bacterial cells yields a protein that binds in vitro to both the CCAAT homology and the enhancer core homology, providing conclusive evidence that a single gene product accounts for both binding activities. By examining the properties of protease-derived fragments of C/EBP, we have localized its DNA-binding domain to a 14-kD fragment. A 60-amino-acid segment located within the DNA-binding domain of C/EBP bears sequence similarity to the products of the myc and fos oncogenes.

Original languageEnglish (US)
Pages (from-to)786-800
Number of pages15
JournalGenes & development
Volume2
Issue number7
StatePublished - Jul 1988

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Clone Cells
myc Genes
DNA
DNA-Binding Proteins
Genes
Proteins
Peptide Hydrolases
Hot Temperature
Amino Acids
Liver
In Vitro Techniques

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Landschulz, W. H., Johnson, P. F., Adashi, E. Y., Graves, B. J., & McKnight, S. L. (1988). Isolation of a recombinant copy of the gene encoding C/EBP. Genes & development, 2(7), 786-800.

Isolation of a recombinant copy of the gene encoding C/EBP. / Landschulz, W. H.; Johnson, P. F.; Adashi, E. Y.; Graves, B. J.; McKnight, S. L.

In: Genes & development, Vol. 2, No. 7, 07.1988, p. 786-800.

Research output: Contribution to journalArticle

Landschulz, WH, Johnson, PF, Adashi, EY, Graves, BJ & McKnight, SL 1988, 'Isolation of a recombinant copy of the gene encoding C/EBP.', Genes & development, vol. 2, no. 7, pp. 786-800.
Landschulz WH, Johnson PF, Adashi EY, Graves BJ, McKnight SL. Isolation of a recombinant copy of the gene encoding C/EBP. Genes & development. 1988 Jul;2(7):786-800.
Landschulz, W. H. ; Johnson, P. F. ; Adashi, E. Y. ; Graves, B. J. ; McKnight, S. L. / Isolation of a recombinant copy of the gene encoding C/EBP. In: Genes & development. 1988 ; Vol. 2, No. 7. pp. 786-800.
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