Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells

James T. Drummond, Guo Min Li, Matthew J. Longley, Paul Modrich

Research output: Contribution to journalArticle

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Abstract

A mismatch-binding heterodimer of hMSH2 and a 160-kilodalton polypeptide has been isolated from HeLa cells by virtue of its ability to restore mismatch repair to nuclear extracts of hMSH2-deficient LoVo colorectal tumor cells. This heterodimer, designated hMutSα, also restores mismatch repair to extracts of alkylation-tolerant MT1 lymphoblastoid cells and HCT-15 colorectal tumor cells, which are selectively defective in the repair of base-base and single-nucleotide insertion-deletion mismatches. Because HCT-15 cells appear to be free of hMSH2 mutations, this selective repair defect is likely a result of a deficiency of the hMutSα 160-kilodalton subunit, and mutations in the corresponding gene may confer hypermutability and cancer predisposition.

Original languageEnglish (US)
Pages (from-to)1909-1912
Number of pages4
JournalScience
Volume268
Issue number5219
StatePublished - Jan 1 1995

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DNA Mismatch Repair
Colorectal Neoplasms
Neoplasms
Mutation
Alkylation
HeLa Cells
Nucleotides
Peptides
Genes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Drummond, J. T., Li, G. M., Longley, M. J., & Modrich, P. (1995). Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. Science, 268(5219), 1909-1912.

Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. / Drummond, James T.; Li, Guo Min; Longley, Matthew J.; Modrich, Paul.

In: Science, Vol. 268, No. 5219, 01.01.1995, p. 1909-1912.

Research output: Contribution to journalArticle

Drummond, JT, Li, GM, Longley, MJ & Modrich, P 1995, 'Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells', Science, vol. 268, no. 5219, pp. 1909-1912.
Drummond JT, Li GM, Longley MJ, Modrich P. Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. Science. 1995 Jan 1;268(5219):1909-1912.
Drummond, James T. ; Li, Guo Min ; Longley, Matthew J. ; Modrich, Paul. / Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. In: Science. 1995 ; Vol. 268, No. 5219. pp. 1909-1912.
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