Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease

John M. Chirgwin; Alan E. Przybyla; Raymond J. MacDonald; William J. Rutter

doi:10.1021/bi00591a005

Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease

John M. Chirgwin, Alan E. Przybyla, Raymond J. MacDonald, William J. Rutter

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Abstract

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.

Original language	English (US)
Pages (from-to)	5294-5299
Number of pages	6
Journal	Biochemistry
Volume	18
Issue number	24
DOIs	https://doi.org/10.1021/bi00591a005
State	Published - 1979

ASJC Scopus subject areas

Biochemistry

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abstract = "Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.",

author = "Chirgwin, {John M.} and Przybyla, {Alan E.} and MacDonald, {Raymond J.} and Rutter, {William J.}",

year = "1979",

doi = "10.1021/bi00591a005",

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volume = "18",

pages = "5294--5299",

journal = "Biochemistry",

issn = "0006-2960",

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T1 - Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease

AU - Chirgwin, John M.

AU - Przybyla, Alan E.

AU - MacDonald, Raymond J.

AU - Rutter, William J.

PY - 1979

Y1 - 1979

N2 - Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.

AB - Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.

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JO - Biochemistry

JF - Biochemistry

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ER -

Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease

Abstract

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