Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

Yucheng Zeng, Mark A. Lehrman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.

Original languageEnglish (US)
Pages (from-to)266-271
Number of pages6
JournalAnalytical Biochemistry
Volume193
Issue number2
DOIs
StatePublished - 1991

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Asparagine
Cricetulus
Polysaccharides
Ovary
Cells
Cell Line
Animals
Mutation
mannosyl(3)-N-acetyl(2)glucose
mannosyl(5)-N-acetyl(2)-glucose diphosphate dolichol
alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans. / Zeng, Yucheng; Lehrman, Mark A.

In: Analytical Biochemistry, Vol. 193, No. 2, 1991, p. 266-271.

Research output: Contribution to journalArticle

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