Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

Yucheng Zeng; Mark A. Lehrman

doi:10.1016/0003-2697(91)90020-T

Isolation of Chinese hamster ovary cell lines producing Man₃GlcNAc₂ asparagine-linked glycans

Yucheng Zeng, Mark A. Lehrman

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Chinese hamster ovary lines with two mutations, one causing accumulation of Man₅GlcNAc₂-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man₃GlcNAc₂. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.

Original language	English (US)
Pages (from-to)	266-271
Number of pages	6
Journal	Analytical biochemistry
Volume	193
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(91)90020-T
State	Published - Mar 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans",

abstract = "Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.",

author = "Yucheng Zeng and Lehrman, {Mark A.}",

note = "Funding Information: This work was done during the tenure of an Established Investiga-torship award from the American Heart Association and Sandoz Pharmaceuticals Corporation, and was supported by Grants GM38545 from the National Institutes of Health and 87-A-105 from the Searle Scholars Program/Chicago Community Trust, awarded to M.A.L. The manuscript was prepared with the skillful assistance of Diane Cali.",

year = "1991",

month = mar,

doi = "10.1016/0003-2697(91)90020-T",

language = "English (US)",

volume = "193",

pages = "266--271",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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T1 - Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

AU - Zeng, Yucheng

AU - Lehrman, Mark A.

N1 - Funding Information: This work was done during the tenure of an Established Investiga-torship award from the American Heart Association and Sandoz Pharmaceuticals Corporation, and was supported by Grants GM38545 from the National Institutes of Health and 87-A-105 from the Searle Scholars Program/Chicago Community Trust, awarded to M.A.L. The manuscript was prepared with the skillful assistance of Diane Cali.

PY - 1991/3

Y1 - 1991/3

N2 - Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.

AB - Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.

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Isolation of Chinese hamster ovary cell lines producing Man₃GlcNAc₂ asparagine-linked glycans

Abstract

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