Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans

Yucheng Zeng, Mark A. Lehrman

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Abstract

Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.

Original languageEnglish (US)
Pages (from-to)266-271
Number of pages6
JournalAnalytical biochemistry
Volume193
Issue number2
DOIs
StatePublished - Mar 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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