Isolation of cytochrome bc1 complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum

R. Max Wynn, Dale F. Gaul, Won Ki Choi, Robert W. Shaw, David B. Knaff

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21 Scopus citations

Abstract

Cytochrome bc1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Both complexes are largely free of bacteriochlorophyll and carotenoids and contain cytochromes b and c1 in a 2:1 molar ratio. For the Rps. viridis complex, evidence has been obtained for two spectrally distinct b-cytochromes. The R. rubrum complex contains a Rieske iron-sulfur protein (present in approximately 1:1 molar ratio to cytochrome c1) and catalyzes an antimycin A- and myxothiazol-sensitive electron transfer from duroquinol to equine cytochrome c or R. rubrum cytochrome c2. Although an attempt to prepare a cytochrome bc1 complex from the gliding green bacterium Chloroflexus aurantiacus was not successful, membranes isolated from phototrophically grown Cfl. aurantiacus were shown to contain a Rieske iron-sulfur protein and protoheme (the prosthetic group of b-type cytochromes).

Original languageEnglish (US)
Pages (from-to)181-195
Number of pages15
JournalPhotosynthesis Research
Volume9
Issue number1-2
DOIs
Publication statusPublished - Jan 1986

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Keywords

  • cytochrome bc complex
  • photosynthetic bacteria
  • Rieske iron-sulfur protein

ASJC Scopus subject areas

  • Plant Science

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