Isolation of cytochrome bc1 complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum

R. Max Wynn, Dale F. Gaul, Won Ki Choi, Robert W. Shaw, David B. Knaff

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Cytochrome bc1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Both complexes are largely free of bacteriochlorophyll and carotenoids and contain cytochromes b and c1 in a 2:1 molar ratio. For the Rps. viridis complex, evidence has been obtained for two spectrally distinct b-cytochromes. The R. rubrum complex contains a Rieske iron-sulfur protein (present in approximately 1:1 molar ratio to cytochrome c1) and catalyzes an antimycin A- and myxothiazol-sensitive electron transfer from duroquinol to equine cytochrome c or R. rubrum cytochrome c2. Although an attempt to prepare a cytochrome bc1 complex from the gliding green bacterium Chloroflexus aurantiacus was not successful, membranes isolated from phototrophically grown Cfl. aurantiacus were shown to contain a Rieske iron-sulfur protein and protoheme (the prosthetic group of b-type cytochromes).

Original languageEnglish (US)
Pages (from-to)181-195
Number of pages15
JournalPhotosynthesis Research
Issue number1-2
StatePublished - Jan 1 1986


  • Rieske iron-sulfur protein
  • cytochrome bc complex
  • photosynthetic bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology


Dive into the research topics of 'Isolation of cytochrome bc<sub>1</sub> complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum'. Together they form a unique fingerprint.

Cite this