Isolation of MEK5 and differential expression of alternatively spliced forms

J. M. English, C. A. Vanderbilt, S. Xu, S. Marcus, M. H. Cobb

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

The prototype mitogen-activated protein (MAP) kinase module is a three- kinase cascade consisting of the MAP kinase, extracellular signal-regulated protein kinase (ERK) 1 or ERK2, the MAP/ERK kinase (MEK) MEK1 or MEK2, and the MEK kinase, Raf-1 or B-Raf. This and other MAP kinase modules are thought to be critical signal transducers in major cellular events including proliferation, differentiation, and stress responses. To identify novel mammalian MAP kinase modules, polymerase chain reaction was used to isolate a new MEK family member, MEK5, from the rat. MEK5 is more closely related to MEK1 and MEK2 than to the other known mammalian MEKs, MKK3 and MKK4. MEK5 is thought to lie in an uncharacterized MAP kinase pathway, because MEK5 does not phosphorylate the ERK/MAP kinase family members ERK1, ERK2, ERK3, JNK/SAPK, or p38/HOG1, nor will Raf-1, c-Mos, or MEKK1 highly phosphorylate it. Alternative splicing results in a 50-kDa α and a 40-kDa β isoform of MEK5. MEK5β is ubiquitously distributed and primarily cytosolic. MEK5α is expressed most highly in liver and brain and is particulate. The 23 amino acids encoded by the 5' exon in the larger α isoform are similar to a sequence found in certain proteins believed to associate with the actin cytoskeleton; this alternatively spliced modular domain may lead to the differential subcellular localization of MEK5α.

Original languageEnglish (US)
Pages (from-to)28897-28902
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number48
DOIs
StatePublished - 1995

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Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase Kinases
Protein Isoforms
Phosphotransferases
Proto-Oncogene Proteins c-raf
MAP Kinase Kinase Kinase 1
Polymerase chain reaction
Alternative Splicing
Mitogens
Extracellular Signal-Regulated MAP Kinases
Liver
Protein Kinases
p38 Mitogen-Activated Protein Kinases
Rats
Actins
Transducers
Actin Cytoskeleton
Exons
Brain
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation of MEK5 and differential expression of alternatively spliced forms. / English, J. M.; Vanderbilt, C. A.; Xu, S.; Marcus, S.; Cobb, M. H.

In: Journal of Biological Chemistry, Vol. 270, No. 48, 1995, p. 28897-28902.

Research output: Contribution to journalArticle

English, J. M. ; Vanderbilt, C. A. ; Xu, S. ; Marcus, S. ; Cobb, M. H. / Isolation of MEK5 and differential expression of alternatively spliced forms. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 48. pp. 28897-28902.
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