Isolation of Protein Ligands from Large Peptoid Libraries

Prasanna G. Alluri, M. Muralidhar Reddy, Kiran Bachhawat-Sikder, Hernando J. Olivos, Thomas Kodadek

Research output: Contribution to journalArticle

167 Scopus citations

Abstract

The isolation of ligands for large numbers of proteins is an important goal in proteomics. Whereas peptide libraries are rich sources of protein-binding molecules, native peptides have certain undesirable properties, such as sensitivity to proteases that make them less than ideal for some applications. We report here the construction and characterization of large, chemically diverse combinatorial libraries of peptoids (N-substituted oligoglycines). A protocol for the isolation of specific protein-binding molecules from these libraries is described. These data suggest that peptoid libraries will prove to be inexpensive and convenient sources of protein ligands.

Original languageEnglish (US)
Pages (from-to)13995-14004
Number of pages10
JournalJournal of the American Chemical Society
Volume125
Issue number46
DOIs
StatePublished - Nov 19 2003

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Alluri, P. G., Reddy, M. M., Bachhawat-Sikder, K., Olivos, H. J., & Kodadek, T. (2003). Isolation of Protein Ligands from Large Peptoid Libraries. Journal of the American Chemical Society, 125(46), 13995-14004. https://doi.org/10.1021/ja036417x