Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea

S. Zaki Salahuddin, Alan G. Palestine, Ellen Heck, Dharam Ablashi, Martha Luckenbach, James P. McCulley, Robert B. Nussenblatt

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Abstract

Corneoscleral donor tissue from a donor with a positive serum antibody to HTLV-III but without the overt clinical signs of the acquired immune deficiency syndrome (AIDS) was cultured for the presence of the human T-cell leukemia/lymphotropic virus type III (HTLV-III). The virus was isolated from the two corneal specimens in this patient after the tissue had been stored for four days in McCarey-Kaufman medium. The presence of HTLV-111 was confirmed by the detection of viral core proteins (approximately 24,000 protein, termed P24 gag), by immunofluorescence of a touch preparation of the corneal epithelium as well as in cells cultured in vitro. The percentage of immunofluorescent cells detected by HTLV-III anti-P24 antibody ranged between 2% and 3%. These findings emphasize the possibility of transmission of this virus via corneal transplantation surgery. Although no documented cases of AIDS have occurred in corneal transplant recipients, serologic screening of donors before the use of the tissue for transplantation is advisable.

Original languageEnglish (US)
Pages (from-to)149-152
Number of pages4
JournalAmerican Journal of Ophthalmology
Volume101
Issue number2
StatePublished - Feb 15 1986

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Cornea
HIV
Acquired Immunodeficiency Syndrome
Tissue Donors
Viral Core Proteins
Viruses
Donor Selection
Tissue Transplantation
Corneal Epithelium
Corneal Transplantation
Touch
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Cultured Cells
Antibodies
Serum
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Salahuddin, S. Z., Palestine, A. G., Heck, E., Ablashi, D., Luckenbach, M., McCulley, J. P., & Nussenblatt, R. B. (1986). Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea. American Journal of Ophthalmology, 101(2), 149-152.

Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea. / Salahuddin, S. Zaki; Palestine, Alan G.; Heck, Ellen; Ablashi, Dharam; Luckenbach, Martha; McCulley, James P.; Nussenblatt, Robert B.

In: American Journal of Ophthalmology, Vol. 101, No. 2, 15.02.1986, p. 149-152.

Research output: Contribution to journalArticle

Salahuddin, SZ, Palestine, AG, Heck, E, Ablashi, D, Luckenbach, M, McCulley, JP & Nussenblatt, RB 1986, 'Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea', American Journal of Ophthalmology, vol. 101, no. 2, pp. 149-152.
Salahuddin SZ, Palestine AG, Heck E, Ablashi D, Luckenbach M, McCulley JP et al. Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea. American Journal of Ophthalmology. 1986 Feb 15;101(2):149-152.
Salahuddin, S. Zaki ; Palestine, Alan G. ; Heck, Ellen ; Ablashi, Dharam ; Luckenbach, Martha ; McCulley, James P. ; Nussenblatt, Robert B. / Isolation of the human T-cell leukemia/lymphotropic virus type III from the cornea. In: American Journal of Ophthalmology. 1986 ; Vol. 101, No. 2. pp. 149-152.
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