ISOLATION OF THE MHC GENES ENCODING THE TUMOUR‐SPECIFIC CLASS I ANTIGENS EXPRESSED ON A MURINE FIBROSARCOMA

H. J. Stauss, R. Linsk, A. Fischer, S. Watts, D. Banasiak, A. Haberman, I. Clark, J. Forman, M. McMillan, H. Schreiber, R. S. Goodenow

Research output: Contribution to journalArticle

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Abstract

The C3H UV‐induced fibrosarcoma, 1591, is highly immunogenic and, therefore, is readily rejected when transplanted into immunocompetent syngeneic recipients. Previous analysis of 1591 with tumour‐specific or H‐2‐reactive monoclonal antibodies revealed that this antigenicity might be due to the expression of two novel class I major histocompatibility complex (MHC) antigens. In this report we describe the molecular cloning and initial characterization of three genes which account for all of the unique serological class I reactivities observed on this tumour. These include two distinct, but highly conserved, H‐2L‐like genes, and a third gene the product of which bears determinants which are characteristic of both the tumour and of class I products of the H‐2k haplotype. Moreover, each of these genes contains a polymorphic restriction enzyme fragment which is detected in the class I sequences of 1591 relative to normal C3H tissue. Since the expression of these polymorphic class I sequences is relevant to the immunogenicity of 1591, the mutational events by which these genes were generated may be significant to the immunobiology of this tumour.

Original languageEnglish (US)
Pages (from-to)101-112
Number of pages12
JournalInternational Journal of Immunogenetics
Volume13
Issue number2-3
DOIs
StatePublished - Jan 1 1986

Fingerprint

Histocompatibility Antigens Class I
Fibrosarcoma
Major Histocompatibility Complex
Genes
Neoplasms
Molecular Cloning
Haplotypes
Monoclonal Antibodies
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

ISOLATION OF THE MHC GENES ENCODING THE TUMOUR‐SPECIFIC CLASS I ANTIGENS EXPRESSED ON A MURINE FIBROSARCOMA. / Stauss, H. J.; Linsk, R.; Fischer, A.; Watts, S.; Banasiak, D.; Haberman, A.; Clark, I.; Forman, J.; McMillan, M.; Schreiber, H.; Goodenow, R. S.

In: International Journal of Immunogenetics, Vol. 13, No. 2-3, 01.01.1986, p. 101-112.

Research output: Contribution to journalArticle

Stauss, HJ, Linsk, R, Fischer, A, Watts, S, Banasiak, D, Haberman, A, Clark, I, Forman, J, McMillan, M, Schreiber, H & Goodenow, RS 1986, 'ISOLATION OF THE MHC GENES ENCODING THE TUMOUR‐SPECIFIC CLASS I ANTIGENS EXPRESSED ON A MURINE FIBROSARCOMA', International Journal of Immunogenetics, vol. 13, no. 2-3, pp. 101-112. https://doi.org/10.1111/j.1744-313X.1986.tb01090.x
Stauss, H. J. ; Linsk, R. ; Fischer, A. ; Watts, S. ; Banasiak, D. ; Haberman, A. ; Clark, I. ; Forman, J. ; McMillan, M. ; Schreiber, H. ; Goodenow, R. S. / ISOLATION OF THE MHC GENES ENCODING THE TUMOUR‐SPECIFIC CLASS I ANTIGENS EXPRESSED ON A MURINE FIBROSARCOMA. In: International Journal of Immunogenetics. 1986 ; Vol. 13, No. 2-3. pp. 101-112.
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