Isolation of two functional retinoid X receptor subtypes from the ixodid tick, Amblyomma americanum (L.)

Xiaoping Guo, Qing Xu, Margaret A. Harmon, Xiaojing Jin, Vincent Laudet, David J. Mangelsdorf, Melanie J. Palmer

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Retinoid X receptors (RXR) play a central role in a variety of nuclear signaling pathways in both vertebrates and invertebrates. Vertebrate RXRs are encoded by a multigene family whereas the insect RXR homologue, ultraspiracle (USP), is encoded by a single gene. To determine if acarines possess an RXR homologue similar to insect USPs, we isolated cDNAs encoding two distinct RXR genes, AamRXR1 and AamRXR2, from the ixodid tick, Amblyomma americanum (L.). The DNA binding domains share ≃ 95 and 87% identity, respectively, with DNA binding domains from insect USP and vertebrate RXR proteins. However, the ligand binding domains of the AamRXRs are more similar to vertebrate RXRs than to insect USP ligand binding domains ( ~ 71 vs ~ 52%). Northern blot and RT-PCR analysis reveal both unique and overlapping patterns of AamRXR1 and AamRXR2 expression. Transactivation analysis show that bath AamRXRs encode proteins which can form functional ecdysteroid receptors but are unlikely to bind retinoic acids.

Original languageEnglish (US)
Pages (from-to)45-60
Number of pages16
JournalMolecular and Cellular Endocrinology
Volume139
Issue number1-2
DOIs
StatePublished - Apr 30 1998

Keywords

  • Amblyomma americanum
  • Ecdysteroid
  • RXR
  • Retinoid X receptors
  • Tick

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Isolation of two functional retinoid X receptor subtypes from the ixodid tick, Amblyomma americanum (L.)'. Together they form a unique fingerprint.

  • Cite this