Isozymes of fructose 6-phosphate,2-kinase: Fructose-2,6-bisphosphatase in rat and bovine heart, liver, and skeletal muscle

Matsuo Taniyama, Kazuo Kitamura, Helmut Thomas, J. W R Lawson, Kosaku Uyeda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The distribution of Fructose 6-P,2-kinase:Fructose 2,6-bisphosphatase in rat and bovine heart, liver, and skeletal muscle tissues was examined. With DEAE-cellulose chromatography, two peaks (I and II) of Fru 6-P,2-kinase activity were detected in all tissue extracts. Peak I was the predominant form both in rat and bovine heart tissue, while peak II was the major form in liver and skeletal muscle. Antibodies to heart enzyme reacted specifically with peak I, and antibodies to liver enzyme reacted with peak II from both liver and skeletal muscle. All the isozymes were bifunctional. All the tissues examined contained other isozymes in minor amounts.

Original languageEnglish (US)
Pages (from-to)949-954
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number3
DOIs
StatePublished - Dec 30 1988

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Phosphofructokinase-2
Liver
Isoenzymes
Muscle
Rats
Myocardium
Skeletal Muscle
Tissue
DEAE-Cellulose Chromatography
DEAE-Cellulose
Tissue Extracts
Antibodies
Enzymes
Chromatography
Muscles

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isozymes of fructose 6-phosphate,2-kinase : Fructose-2,6-bisphosphatase in rat and bovine heart, liver, and skeletal muscle. / Taniyama, Matsuo; Kitamura, Kazuo; Thomas, Helmut; Lawson, J. W R; Uyeda, Kosaku.

In: Biochemical and Biophysical Research Communications, Vol. 157, No. 3, 30.12.1988, p. 949-954.

Research output: Contribution to journalArticle

Taniyama, Matsuo ; Kitamura, Kazuo ; Thomas, Helmut ; Lawson, J. W R ; Uyeda, Kosaku. / Isozymes of fructose 6-phosphate,2-kinase : Fructose-2,6-bisphosphatase in rat and bovine heart, liver, and skeletal muscle. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 157, No. 3. pp. 949-954.
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