Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor

Laura B. Sheard, Xu Tan, Haibin Mao, John Withers, Gili Ben-Nissan, Thomas R. Hinds, Yuichi Kobayashi, Fong Fu Hsu, Michal Sharon, John Browse, Sheng Yang He, Jose Rizo-Rey, Gregg A. Howe, Ning Zheng

Research output: Contribution to journalArticle

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Abstract

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R, 7S)-jasmonoyl-l- isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved ±-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

Original languageEnglish (US)
Pages (from-to)400-407
Number of pages8
JournalNature
Volume468
Issue number7322
DOIs
StatePublished - Nov 18 2010

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Inositol Phosphates
F-Box Proteins
Hormones
Repressor Proteins
Aptitude
Plant Growth Regulators
Plant Development
Ubiquitination
Growth and Development
Arabidopsis
jasmonic acid
coronatine
Pharmacology
Ligands

ASJC Scopus subject areas

  • General

Cite this

Sheard, L. B., Tan, X., Mao, H., Withers, J., Ben-Nissan, G., Hinds, T. R., ... Zheng, N. (2010). Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature, 468(7322), 400-407. https://doi.org/10.1038/nature09430

Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. / Sheard, Laura B.; Tan, Xu; Mao, Haibin; Withers, John; Ben-Nissan, Gili; Hinds, Thomas R.; Kobayashi, Yuichi; Hsu, Fong Fu; Sharon, Michal; Browse, John; He, Sheng Yang; Rizo-Rey, Jose; Howe, Gregg A.; Zheng, Ning.

In: Nature, Vol. 468, No. 7322, 18.11.2010, p. 400-407.

Research output: Contribution to journalArticle

Sheard, LB, Tan, X, Mao, H, Withers, J, Ben-Nissan, G, Hinds, TR, Kobayashi, Y, Hsu, FF, Sharon, M, Browse, J, He, SY, Rizo-Rey, J, Howe, GA & Zheng, N 2010, 'Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor', Nature, vol. 468, no. 7322, pp. 400-407. https://doi.org/10.1038/nature09430
Sheard LB, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds TR et al. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature. 2010 Nov 18;468(7322):400-407. https://doi.org/10.1038/nature09430
Sheard, Laura B. ; Tan, Xu ; Mao, Haibin ; Withers, John ; Ben-Nissan, Gili ; Hinds, Thomas R. ; Kobayashi, Yuichi ; Hsu, Fong Fu ; Sharon, Michal ; Browse, John ; He, Sheng Yang ; Rizo-Rey, Jose ; Howe, Gregg A. ; Zheng, Ning. / Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. In: Nature. 2010 ; Vol. 468, No. 7322. pp. 400-407.
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