Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures

Annalisa Tassoni, Silvia Fornalè, Marina Franceschetti, Federica Musiani, Anthony J. Michael, Barry Perry, Nello Bagni

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Here the effect of jasmonic acid, methyljasmonate and Na-orthovanadate on the production of resveratrol was studied in Vitis vinifera cv. Barbera cell suspension cultures. Na-orthovanadate at 0.1 mM and 1 mM concentration was efficient in promoting the production and/or accumulation and release in the culture medium of cis-resveratrol while trans-resveratrol levels were not affected by this treatment. Methyljasmonate was highly effective in stimulating both trans- and cis-resveratrol endogenous accumulation, as well as their release into the culture medium. Cis-resveratrol was absent or detected in very low amounts in the controls. Jasmonic acid was less efficient than methyljasmonate in promoting endogenous resveratrol accumulation, but it stimulated the release in the culture medium especially of cis-resveratrol. Gel analysis was performed on control and 10 μM MeJA treated cell suspensions. Results showed an up-regulation of the stilbene synthase demonstrating that MeJA stimulated the synthesis ex-novo of this protein.

Original languageEnglish (US)
Pages (from-to)895-905
Number of pages11
JournalNew Phytologist
Volume166
Issue number3
DOIs
StatePublished - Jun 2005

Fingerprint

Vanadates
resveratrol
Vitis
jasmonic acid
Cell culture
Vitis vinifera
cell culture
Cell Culture Techniques
Culture Media
culture media
cell suspension culture
Suspensions
gels
Up-Regulation
Gels
Cells
synthesis

Keywords

  • Grapevine
  • Jasmonic acid
  • Methyljasmonate
  • Na-orthovanadate
  • Resveratrol
  • Stilbenes
  • Vitis vinifera

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures. / Tassoni, Annalisa; Fornalè, Silvia; Franceschetti, Marina; Musiani, Federica; Michael, Anthony J.; Perry, Barry; Bagni, Nello.

In: New Phytologist, Vol. 166, No. 3, 06.2005, p. 895-905.

Research output: Contribution to journalArticle

Tassoni, Annalisa ; Fornalè, Silvia ; Franceschetti, Marina ; Musiani, Federica ; Michael, Anthony J. ; Perry, Barry ; Bagni, Nello. / Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures. In: New Phytologist. 2005 ; Vol. 166, No. 3. pp. 895-905.
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