JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction

M. Selgrad, R. De Giorgio, L. Fini, R. F. Cogliandro, S. Williams, V. Stanghellini, G. Barbara, M. Tonini, R. Corinaldesi, R. M. Genta, R. Domiati-Saad, R. Meyer, A. Goel, C. R. Boland, L. Ricciardiello

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Abstract

Background and aim: Chronic idiopathic intestinal pseudo-obstruction (CUP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastrointestinal tract. The hypothesis that JCV infects the myenteric plexuses of patients with CUP was tested. Methods: 10 patients with CllP and 61 normal specimens (30 ascending colon and 31 ileum) from patients with uncomplicated colon cancer were studied. DNA was extracted from the myenteric plexuses, and JCV T antigen (TAg) DNA and the viral regulatory region were detected by PCR and sequencing. Immunohistochemistry was performed to detect JCV viral protein expression, neuronal and glial markers. Fluorescence in situ hybridisation was performed for cellular localisation of the JCV infection. Results: Clinical studies demonstrated neurogenic impairment, and pathological analyses showed neuropathy in each patient with CUP. JCV TAg DNA was found in the myenteric plexuses of 8/10 (80%) of the patients with CllP and 3/31 (9.7%) of the control patients (p<0.001). All samples were JCV Mad-1 strains. Seven of the 10 CUP specimens expressed both JCV TAg and the JCV viral protein VP1, while none of the controls expressed either. JCV infection co-localised with glial fibrillary acidic protein expression, a marker of enteric glial cells. Conclusion: JCV infection occurs in the myenteric plexuses of patients with CllP. The JCV localisation in enteroglial cells suggests a possible pathological role for this virus in enteric neuropathy.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalGut
Volume58
Issue number1
DOIs
StatePublished - Jan 2009

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JC Virus
Intestinal Pseudo-Obstruction
Neuroglia
Myenteric Plexus
Viral Tumor Antigens
Virus Diseases
Viral Proteins
Ascending Colon
Polyomavirus
Gastrointestinal Motility
Nucleic Acid Regulatory Sequences
Glial Fibrillary Acidic Protein
Fluorescence In Situ Hybridization
Ileum
Colonic Neoplasms
Gastrointestinal Tract

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Selgrad, M., De Giorgio, R., Fini, L., Cogliandro, R. F., Williams, S., Stanghellini, V., ... Ricciardiello, L. (2009). JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction. Gut, 58(1), 25-32. https://doi.org/10.1136/gut.2008.152512

JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction. / Selgrad, M.; De Giorgio, R.; Fini, L.; Cogliandro, R. F.; Williams, S.; Stanghellini, V.; Barbara, G.; Tonini, M.; Corinaldesi, R.; Genta, R. M.; Domiati-Saad, R.; Meyer, R.; Goel, A.; Boland, C. R.; Ricciardiello, L.

In: Gut, Vol. 58, No. 1, 01.2009, p. 25-32.

Research output: Contribution to journalArticle

Selgrad, M, De Giorgio, R, Fini, L, Cogliandro, RF, Williams, S, Stanghellini, V, Barbara, G, Tonini, M, Corinaldesi, R, Genta, RM, Domiati-Saad, R, Meyer, R, Goel, A, Boland, CR & Ricciardiello, L 2009, 'JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction', Gut, vol. 58, no. 1, pp. 25-32. https://doi.org/10.1136/gut.2008.152512
Selgrad M, De Giorgio R, Fini L, Cogliandro RF, Williams S, Stanghellini V et al. JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction. Gut. 2009 Jan;58(1):25-32. https://doi.org/10.1136/gut.2008.152512
Selgrad, M. ; De Giorgio, R. ; Fini, L. ; Cogliandro, R. F. ; Williams, S. ; Stanghellini, V. ; Barbara, G. ; Tonini, M. ; Corinaldesi, R. ; Genta, R. M. ; Domiati-Saad, R. ; Meyer, R. ; Goel, A. ; Boland, C. R. ; Ricciardiello, L. / JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction. In: Gut. 2009 ; Vol. 58, No. 1. pp. 25-32.
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abstract = "Background and aim: Chronic idiopathic intestinal pseudo-obstruction (CUP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastrointestinal tract. The hypothesis that JCV infects the myenteric plexuses of patients with CUP was tested. Methods: 10 patients with CllP and 61 normal specimens (30 ascending colon and 31 ileum) from patients with uncomplicated colon cancer were studied. DNA was extracted from the myenteric plexuses, and JCV T antigen (TAg) DNA and the viral regulatory region were detected by PCR and sequencing. Immunohistochemistry was performed to detect JCV viral protein expression, neuronal and glial markers. Fluorescence in situ hybridisation was performed for cellular localisation of the JCV infection. Results: Clinical studies demonstrated neurogenic impairment, and pathological analyses showed neuropathy in each patient with CUP. JCV TAg DNA was found in the myenteric plexuses of 8/10 (80{\%}) of the patients with CllP and 3/31 (9.7{\%}) of the control patients (p<0.001). All samples were JCV Mad-1 strains. Seven of the 10 CUP specimens expressed both JCV TAg and the JCV viral protein VP1, while none of the controls expressed either. JCV infection co-localised with glial fibrillary acidic protein expression, a marker of enteric glial cells. Conclusion: JCV infection occurs in the myenteric plexuses of patients with CllP. The JCV localisation in enteroglial cells suggests a possible pathological role for this virus in enteric neuropathy.",
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AU - Selgrad, M.

AU - De Giorgio, R.

AU - Fini, L.

AU - Cogliandro, R. F.

AU - Williams, S.

AU - Stanghellini, V.

AU - Barbara, G.

AU - Tonini, M.

AU - Corinaldesi, R.

AU - Genta, R. M.

AU - Domiati-Saad, R.

AU - Meyer, R.

AU - Goel, A.

AU - Boland, C. R.

AU - Ricciardiello, L.

PY - 2009/1

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N2 - Background and aim: Chronic idiopathic intestinal pseudo-obstruction (CUP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastrointestinal tract. The hypothesis that JCV infects the myenteric plexuses of patients with CUP was tested. Methods: 10 patients with CllP and 61 normal specimens (30 ascending colon and 31 ileum) from patients with uncomplicated colon cancer were studied. DNA was extracted from the myenteric plexuses, and JCV T antigen (TAg) DNA and the viral regulatory region were detected by PCR and sequencing. Immunohistochemistry was performed to detect JCV viral protein expression, neuronal and glial markers. Fluorescence in situ hybridisation was performed for cellular localisation of the JCV infection. Results: Clinical studies demonstrated neurogenic impairment, and pathological analyses showed neuropathy in each patient with CUP. JCV TAg DNA was found in the myenteric plexuses of 8/10 (80%) of the patients with CllP and 3/31 (9.7%) of the control patients (p<0.001). All samples were JCV Mad-1 strains. Seven of the 10 CUP specimens expressed both JCV TAg and the JCV viral protein VP1, while none of the controls expressed either. JCV infection co-localised with glial fibrillary acidic protein expression, a marker of enteric glial cells. Conclusion: JCV infection occurs in the myenteric plexuses of patients with CllP. The JCV localisation in enteroglial cells suggests a possible pathological role for this virus in enteric neuropathy.

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