Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia

Louis A. Saddic, Sarah M. Nicoloro, Olga T. Gupta, Michael P. Czech, Joshua Gorham, Stanton K. Shernan, Christine E. Seidman, Jon G. Seidman, Sary F. Aranki, Simon C. Body, Timothy P. Fitzgibbons, Jochen D. Muehlschlegel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Omentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has not been well characterized. The purpose of this study is to discern the relationship between the expression pattern of ITLN1 RNA in the human heart and the level of circulating ITLN1 protein in plasma from the same patients following myocardial ischemia. Methods: A large cohort of patients (n = 140) undergoing elective cardiac surgery for aortic valve replacement were enrolled in this study. Plasma and left ventricular biopsy samples were taken at the beginning of cardiopulmonary bypass and after an average of 82 min of ischemic cross clamp time. The localization of ITLN1 in epicardial adipose tissue (EAT) was also further characterized with immunoassays and cell fate transition studies. Results: mRNA expression of ITLN1 decreases in left ventricular tissue after acute ischemia in human patients (mean difference 280.48, p = 0.001) whereas plasma protein levels of ITLN1 increase (mean difference 5.24, p < 0.001). Immunohistochemistry localized ITLN1 to the mesothelium or visceral pericardium of EAT. Epithelial to mesenchymal transition in mesothelial cells leads to a downregulation of ITLN1 expression. Conclusions: Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels. These changes may in part be due to an epithelial to mesenchymal transition of the cells that express ITLN1 following ischemia. Trial Registration Clinicaltrials.gov ID: NCT00985049.

Original languageEnglish (US)
Article number87
JournalCardiovascular Diabetology
Volume16
Issue number1
DOIs
StatePublished - Jul 7 2017

Fingerprint

Myocardial Ischemia
Epithelial-Mesenchymal Transition
Adipose Tissue
Blood Proteins
Ischemia
Adipokines
Pericardium
Wounds and Injuries
Cardiopulmonary Bypass
Aortic Valve
Immunoassay
Thoracic Surgery
Coronary Artery Disease
Down-Regulation
Epithelium
Obesity
Immunohistochemistry
RNA
Biopsy
Messenger RNA

Keywords

  • Adipokine
  • Cardiovascular
  • Ischemia
  • Omentin
  • RNA-seq

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Cardiology and Cardiovascular Medicine

Cite this

Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia. / Saddic, Louis A.; Nicoloro, Sarah M.; Gupta, Olga T.; Czech, Michael P.; Gorham, Joshua; Shernan, Stanton K.; Seidman, Christine E.; Seidman, Jon G.; Aranki, Sary F.; Body, Simon C.; Fitzgibbons, Timothy P.; Muehlschlegel, Jochen D.

In: Cardiovascular Diabetology, Vol. 16, No. 1, 87, 07.07.2017.

Research output: Contribution to journalArticle

Saddic, LA, Nicoloro, SM, Gupta, OT, Czech, MP, Gorham, J, Shernan, SK, Seidman, CE, Seidman, JG, Aranki, SF, Body, SC, Fitzgibbons, TP & Muehlschlegel, JD 2017, 'Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia', Cardiovascular Diabetology, vol. 16, no. 1, 87. https://doi.org/10.1186/s12933-017-0567-x
Saddic, Louis A. ; Nicoloro, Sarah M. ; Gupta, Olga T. ; Czech, Michael P. ; Gorham, Joshua ; Shernan, Stanton K. ; Seidman, Christine E. ; Seidman, Jon G. ; Aranki, Sary F. ; Body, Simon C. ; Fitzgibbons, Timothy P. ; Muehlschlegel, Jochen D. / Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia. In: Cardiovascular Diabetology. 2017 ; Vol. 16, No. 1.
@article{447308e56fd84c1985f964c676203836,
title = "Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia",
abstract = "Background: Omentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has not been well characterized. The purpose of this study is to discern the relationship between the expression pattern of ITLN1 RNA in the human heart and the level of circulating ITLN1 protein in plasma from the same patients following myocardial ischemia. Methods: A large cohort of patients (n = 140) undergoing elective cardiac surgery for aortic valve replacement were enrolled in this study. Plasma and left ventricular biopsy samples were taken at the beginning of cardiopulmonary bypass and after an average of 82 min of ischemic cross clamp time. The localization of ITLN1 in epicardial adipose tissue (EAT) was also further characterized with immunoassays and cell fate transition studies. Results: mRNA expression of ITLN1 decreases in left ventricular tissue after acute ischemia in human patients (mean difference 280.48, p = 0.001) whereas plasma protein levels of ITLN1 increase (mean difference 5.24, p < 0.001). Immunohistochemistry localized ITLN1 to the mesothelium or visceral pericardium of EAT. Epithelial to mesenchymal transition in mesothelial cells leads to a downregulation of ITLN1 expression. Conclusions: Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels. These changes may in part be due to an epithelial to mesenchymal transition of the cells that express ITLN1 following ischemia. Trial Registration Clinicaltrials.gov ID: NCT00985049.",
keywords = "Adipokine, Cardiovascular, Ischemia, Omentin, RNA-seq",
author = "Saddic, {Louis A.} and Nicoloro, {Sarah M.} and Gupta, {Olga T.} and Czech, {Michael P.} and Joshua Gorham and Shernan, {Stanton K.} and Seidman, {Christine E.} and Seidman, {Jon G.} and Aranki, {Sary F.} and Body, {Simon C.} and Fitzgibbons, {Timothy P.} and Muehlschlegel, {Jochen D.}",
year = "2017",
month = "7",
day = "7",
doi = "10.1186/s12933-017-0567-x",
language = "English (US)",
volume = "16",
journal = "Cardiovascular Diabetology",
issn = "1475-2840",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia

AU - Saddic, Louis A.

AU - Nicoloro, Sarah M.

AU - Gupta, Olga T.

AU - Czech, Michael P.

AU - Gorham, Joshua

AU - Shernan, Stanton K.

AU - Seidman, Christine E.

AU - Seidman, Jon G.

AU - Aranki, Sary F.

AU - Body, Simon C.

AU - Fitzgibbons, Timothy P.

AU - Muehlschlegel, Jochen D.

PY - 2017/7/7

Y1 - 2017/7/7

N2 - Background: Omentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has not been well characterized. The purpose of this study is to discern the relationship between the expression pattern of ITLN1 RNA in the human heart and the level of circulating ITLN1 protein in plasma from the same patients following myocardial ischemia. Methods: A large cohort of patients (n = 140) undergoing elective cardiac surgery for aortic valve replacement were enrolled in this study. Plasma and left ventricular biopsy samples were taken at the beginning of cardiopulmonary bypass and after an average of 82 min of ischemic cross clamp time. The localization of ITLN1 in epicardial adipose tissue (EAT) was also further characterized with immunoassays and cell fate transition studies. Results: mRNA expression of ITLN1 decreases in left ventricular tissue after acute ischemia in human patients (mean difference 280.48, p = 0.001) whereas plasma protein levels of ITLN1 increase (mean difference 5.24, p < 0.001). Immunohistochemistry localized ITLN1 to the mesothelium or visceral pericardium of EAT. Epithelial to mesenchymal transition in mesothelial cells leads to a downregulation of ITLN1 expression. Conclusions: Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels. These changes may in part be due to an epithelial to mesenchymal transition of the cells that express ITLN1 following ischemia. Trial Registration Clinicaltrials.gov ID: NCT00985049.

AB - Background: Omentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has not been well characterized. The purpose of this study is to discern the relationship between the expression pattern of ITLN1 RNA in the human heart and the level of circulating ITLN1 protein in plasma from the same patients following myocardial ischemia. Methods: A large cohort of patients (n = 140) undergoing elective cardiac surgery for aortic valve replacement were enrolled in this study. Plasma and left ventricular biopsy samples were taken at the beginning of cardiopulmonary bypass and after an average of 82 min of ischemic cross clamp time. The localization of ITLN1 in epicardial adipose tissue (EAT) was also further characterized with immunoassays and cell fate transition studies. Results: mRNA expression of ITLN1 decreases in left ventricular tissue after acute ischemia in human patients (mean difference 280.48, p = 0.001) whereas plasma protein levels of ITLN1 increase (mean difference 5.24, p < 0.001). Immunohistochemistry localized ITLN1 to the mesothelium or visceral pericardium of EAT. Epithelial to mesenchymal transition in mesothelial cells leads to a downregulation of ITLN1 expression. Conclusions: Myocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels. These changes may in part be due to an epithelial to mesenchymal transition of the cells that express ITLN1 following ischemia. Trial Registration Clinicaltrials.gov ID: NCT00985049.

KW - Adipokine

KW - Cardiovascular

KW - Ischemia

KW - Omentin

KW - RNA-seq

UR - http://www.scopus.com/inward/record.url?scp=85021965489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021965489&partnerID=8YFLogxK

U2 - 10.1186/s12933-017-0567-x

DO - 10.1186/s12933-017-0567-x

M3 - Article

VL - 16

JO - Cardiovascular Diabetology

JF - Cardiovascular Diabetology

SN - 1475-2840

IS - 1

M1 - 87

ER -