jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun

Jochen Schütte, Jean Viallet, Marion Nau, Shoshana Segal, Joseph Fedorko, John Minna

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

We have cloned the human jun-B gene and determined its sequence and transforming and trans-activating activities. jun-B is less potent than c-jun in transforming and immortalizing primary rat embryo cells in cooperation with activated ras (effects enhanced by c-fos and TPA); unlike c-jun, jun-B does not transform Rat-1A cells alone. However, cotransfection of c-jun and jun-B into primary rat embryo cells with c-Ha-ras results in a significant decrease in transformation compared with c-jun alone, an event reversed by TPA. Cotransfection of c-jun and jun-B with or without c-fos into F9 teratocarcinoma cells results in decreased trans-activation of AP-1 compared with either gene alone. Introduction of jun-B into primary rat c-jun/ras transformants or c-jun into jun-B/ras transformants also results in a decrease in trans-activation. These findings demonstrate that, whereas jun-B and c-jun each participate in AP-1 trans-activation and malignant transformation, interactions between them involve negative regulation.

Original languageEnglish (US)
Pages (from-to)987-997
Number of pages11
JournalCell
Volume59
Issue number6
DOIs
StatePublished - Dec 22 1989

Fingerprint

Rats
Chemical activation
Transcription Factor AP-1
Embryonic Structures
Genes
jun Genes
Teratocarcinoma

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun. / Schütte, Jochen; Viallet, Jean; Nau, Marion; Segal, Shoshana; Fedorko, Joseph; Minna, John.

In: Cell, Vol. 59, No. 6, 22.12.1989, p. 987-997.

Research output: Contribution to journalArticle

Schütte, Jochen ; Viallet, Jean ; Nau, Marion ; Segal, Shoshana ; Fedorko, Joseph ; Minna, John. / jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun. In: Cell. 1989 ; Vol. 59, No. 6. pp. 987-997.
@article{8d895f27a5a946a285eb7af552e02f66,
title = "jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun",
abstract = "We have cloned the human jun-B gene and determined its sequence and transforming and trans-activating activities. jun-B is less potent than c-jun in transforming and immortalizing primary rat embryo cells in cooperation with activated ras (effects enhanced by c-fos and TPA); unlike c-jun, jun-B does not transform Rat-1A cells alone. However, cotransfection of c-jun and jun-B into primary rat embryo cells with c-Ha-ras results in a significant decrease in transformation compared with c-jun alone, an event reversed by TPA. Cotransfection of c-jun and jun-B with or without c-fos into F9 teratocarcinoma cells results in decreased trans-activation of AP-1 compared with either gene alone. Introduction of jun-B into primary rat c-jun/ras transformants or c-jun into jun-B/ras transformants also results in a decrease in trans-activation. These findings demonstrate that, whereas jun-B and c-jun each participate in AP-1 trans-activation and malignant transformation, interactions between them involve negative regulation.",
author = "Jochen Sch{\"u}tte and Jean Viallet and Marion Nau and Shoshana Segal and Joseph Fedorko and John Minna",
year = "1989",
month = "12",
day = "22",
doi = "10.1016/0092-8674(89)90755-1",
language = "English (US)",
volume = "59",
pages = "987--997",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun

AU - Schütte, Jochen

AU - Viallet, Jean

AU - Nau, Marion

AU - Segal, Shoshana

AU - Fedorko, Joseph

AU - Minna, John

PY - 1989/12/22

Y1 - 1989/12/22

N2 - We have cloned the human jun-B gene and determined its sequence and transforming and trans-activating activities. jun-B is less potent than c-jun in transforming and immortalizing primary rat embryo cells in cooperation with activated ras (effects enhanced by c-fos and TPA); unlike c-jun, jun-B does not transform Rat-1A cells alone. However, cotransfection of c-jun and jun-B into primary rat embryo cells with c-Ha-ras results in a significant decrease in transformation compared with c-jun alone, an event reversed by TPA. Cotransfection of c-jun and jun-B with or without c-fos into F9 teratocarcinoma cells results in decreased trans-activation of AP-1 compared with either gene alone. Introduction of jun-B into primary rat c-jun/ras transformants or c-jun into jun-B/ras transformants also results in a decrease in trans-activation. These findings demonstrate that, whereas jun-B and c-jun each participate in AP-1 trans-activation and malignant transformation, interactions between them involve negative regulation.

AB - We have cloned the human jun-B gene and determined its sequence and transforming and trans-activating activities. jun-B is less potent than c-jun in transforming and immortalizing primary rat embryo cells in cooperation with activated ras (effects enhanced by c-fos and TPA); unlike c-jun, jun-B does not transform Rat-1A cells alone. However, cotransfection of c-jun and jun-B into primary rat embryo cells with c-Ha-ras results in a significant decrease in transformation compared with c-jun alone, an event reversed by TPA. Cotransfection of c-jun and jun-B with or without c-fos into F9 teratocarcinoma cells results in decreased trans-activation of AP-1 compared with either gene alone. Introduction of jun-B into primary rat c-jun/ras transformants or c-jun into jun-B/ras transformants also results in a decrease in trans-activation. These findings demonstrate that, whereas jun-B and c-jun each participate in AP-1 trans-activation and malignant transformation, interactions between them involve negative regulation.

UR - http://www.scopus.com/inward/record.url?scp=0024800229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024800229&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(89)90755-1

DO - 10.1016/0092-8674(89)90755-1

M3 - Article

C2 - 2513129

AN - SCOPUS:0024800229

VL - 59

SP - 987

EP - 997

JO - Cell

JF - Cell

SN - 0092-8674

IS - 6

ER -