Jun-Fos and receptors for vitamins A and D recognize a common response element in the human osteocalcin gene

Roland Schüle, Kazuhiko Umesono, David J. Mangelsdorf, Jack Bolado, J. Wesley Pike, Ronald M. Evans

Research output: Contribution to journalArticle

407 Scopus citations

Abstract

We present evidence that the vitamin D response element in the human osteocalcin gene confers responsiveness to the vitamin A metabolite, retinoic acid. Retinoic acid receptor (RAR) expressed in E. coli binds to this sequence in vitro. Transfection of RAR expression vectors in cultured cells activates heterologous promoters containing this sequence in vivo. This response element contains a consensus AP-1 site TGACTCA and in vitro is bound by the Jun-Fos complex. Unexpectedly, cotransfection of Jun and Fos expression vectors suppresses basal level transcription of the osteocalcin gene and suppresses induction by both retinoic acid and vitamin D3. Additional studies delimit an 11 nucleotide segment as a minimal hormone response element containing the AP-1 site as its core. These results indicate that two distinct classes of transcription factors can recognize common regulatory sequences, a phenomenon we refer to as cross-coupling.

Original languageEnglish (US)
Pages (from-to)497-504
Number of pages8
JournalCell
Volume61
Issue number3
DOIs
StatePublished - May 4 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Jun-Fos and receptors for vitamins A and D recognize a common response element in the human osteocalcin gene'. Together they form a unique fingerprint.

  • Cite this