Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster: a putative juvenile hormone in the higher Diptera.

D. S. Richard, S. W. Applebaum, T. J. Sliter, F. C. Baker, D. A. Schooley, C. C. Reuter, V. C. Henrich, L. I. Gilbert

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Abstract

The in vitro production of juvenile hormone (JH) was investigated by using isolated ring glands from third instar Drosophila melanogaster. A JH-like molecule is secreted that comigrates with a synthetic sample of methyl 6,7;10,11-bisepoxy-3,7,11-trimethyl-(2E)-dodecenoate (JHB3) during TLC, liquid chromatography, and GC analysis. Purified product from farnesoic acid-stimulated ring glands was analyzed by electron impact GC/MS and gave a mass spectrum identical to synthetic JHB3. Additional structure confirmation was obtained following conversion of product from unstimulated biosynthesis to a derivative that comigrated on liquid chromatography with the derivative prepared from synthetic JHB3. Physiological studies revealed that JHB3 is produced solely by the corpus allatum portion of the ring gland in vitro. Isolated ring glands from other cyclorrhaphous dipteran larvae also produce JHB3 almost exclusively in vitro. Corpora allata from mosquito larvae, however, produce only JH III, indicating that JHB3 production may be restricted to the higher Diptera. Topically applied synthetic JHB3 caused developmental responses in newly formed D. melanogaster white puparia similar to those obtained with JH III. The data suggest that JHB3 is a fly juvenile hormone.

Original languageEnglish (US)
Pages (from-to)1421-1425
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number4
StatePublished - Feb 1989

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Juvenile Hormones
Drosophila melanogaster
Diptera
Corpora Allata
Liquid Chromatography
Larva
In Vitro Techniques
methyl 6,7-10,11-bis(epoxy)-3,7,11-trimethyl-2-dodecenoate
Culicidae
Electrons

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster : a putative juvenile hormone in the higher Diptera. / Richard, D. S.; Applebaum, S. W.; Sliter, T. J.; Baker, F. C.; Schooley, D. A.; Reuter, C. C.; Henrich, V. C.; Gilbert, L. I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 4, 02.1989, p. 1421-1425.

Research output: Contribution to journalArticle

Richard, D. S. ; Applebaum, S. W. ; Sliter, T. J. ; Baker, F. C. ; Schooley, D. A. ; Reuter, C. C. ; Henrich, V. C. ; Gilbert, L. I. / Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster : a putative juvenile hormone in the higher Diptera. In: Proceedings of the National Academy of Sciences of the United States of America. 1989 ; Vol. 86, No. 4. pp. 1421-1425.
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