Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus

Katherine E. Süel, Yuh Min Chook

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A previous bioinformatics study identified a putative PY-NLS in the yeast transcription factor Tfg2p (Suel, K. E., Gu, H., and Chook, Y. M. (2008) PLoS Biol. 6, e137). In this study, we validate Tfg2p as a Kap104p substrate and examine the energetic organization of its PY-NLS. The Tfg2p PY-NLS can target a heterologous protein into the cell nucleus through interactions with Kap104p. Surprisingly, full-length Tfg2p is still localized to the nucleus of Kap104p temperature-sensitive cells and, similarly, Tfg2p with a mutated PY-NLS is nuclear in wild-type cells. Other Karyopherinßs (Kapßs) such as Kap108p and Kap120p also bind Tfg2p and may import it into the nucleus. More importantly, we demonstrate that Tfg2p is retained in the nucleus through DNA binding. Mutations of DNA binding residues relieve nuclear retention and unmask the role of Kap104p in Tfg2p nuclear import. More generally, steady-state localization of a nuclear protein is dictated by its nuclear import and export activities as well as its interactions in the nucleus and the cytoplasm.

Original languageEnglish (US)
Pages (from-to)15416-15424
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number23
DOIs
StatePublished - Jun 5 2009

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Cell Nucleus Active Transport
Transcription Factors
Karyopherins
DNA
Bioinformatics
Nuclear Proteins
Yeast
Cells
Computational Biology
Cell Nucleus
Cytoplasm
Substrates
Yeasts
Mutation
Proteins
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus. / Süel, Katherine E.; Chook, Yuh Min.

In: Journal of Biological Chemistry, Vol. 284, No. 23, 05.06.2009, p. 15416-15424.

Research output: Contribution to journalArticle

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