Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease

Jillian M. Richmond, Dinesh S. Bangari, Kingsley I. Essien, Sharif D. Currimbhoy, Joanna R. Groom, Amit G. Pandya, Michele E. Youd, Andrew D. Luster, John E. Harris

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Vitiligo is an autoimmune disease of the skin that results in the destruction of melanocytes and the clinical appearance of white spots. Disease pathogenesis depends on IFN-γ and IFN-γ–induced chemokines to promote T-cell recruitment to the epidermis where melanocytes reside. The skin is a complex organ, with a variety of resident cell types. We sought to better define the microenvironment and distinct cellular contributions during autoimmunity in vitiligo, and we found that the epidermis is a chemokine-high niche in both a mouse model and human vitiligo. Analysis of chemokine expression in mouse skin showed that CXCL9 and CXCL10 expression strongly correlate with disease activity, whereas CXCL10 alone correlates with severity, supporting them as potential biomarkers for following disease progression. Further studies in both our mouse model and human patients showed that keratinocytes were the major chemokine producers throughout the course of disease, and functional studies using a conditional signal transducer and activator of transcription (STAT)-1 knockout mouse showed that IFN-γ signaling in keratinocytes was critical for disease progression and proper autoreactive T-cell homing to the epidermis. In contrast, epidermal immune cell populations including endogenous T cells, Langerhans cells, and γδ T cells were not required. These results have important clinical implications, because topical therapies that target IFN-γ signaling in keratinocytes could be safe and effective new treatments, and skin expression of these chemokines could be used to monitor disease activity and treatment responses.

Original languageEnglish (US)
Pages (from-to)350-358
Number of pages9
JournalJournal of Investigative Dermatology
Volume137
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Vitiligo
T-cells
Biomarkers
Chemokines
Epidermis
T-Lymphocytes
Keratinocytes
Skin
Melanocytes
Disease Progression
STAT1 Transcription Factor
Cellular Microenvironment
Langerhans Cells
Dental Caries
Autoimmunity
Knockout Mice
Autoimmune Diseases
Therapeutics
keratinocyte-derived chemokines
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease. / Richmond, Jillian M.; Bangari, Dinesh S.; Essien, Kingsley I.; Currimbhoy, Sharif D.; Groom, Joanna R.; Pandya, Amit G.; Youd, Michele E.; Luster, Andrew D.; Harris, John E.

In: Journal of Investigative Dermatology, Vol. 137, No. 2, 01.02.2017, p. 350-358.

Research output: Contribution to journalArticle

Richmond, Jillian M. ; Bangari, Dinesh S. ; Essien, Kingsley I. ; Currimbhoy, Sharif D. ; Groom, Joanna R. ; Pandya, Amit G. ; Youd, Michele E. ; Luster, Andrew D. ; Harris, John E. / Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease. In: Journal of Investigative Dermatology. 2017 ; Vol. 137, No. 2. pp. 350-358.
@article{182ee5057b4d45d6af549e906549003f,
title = "Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease",
abstract = "Vitiligo is an autoimmune disease of the skin that results in the destruction of melanocytes and the clinical appearance of white spots. Disease pathogenesis depends on IFN-γ and IFN-γ–induced chemokines to promote T-cell recruitment to the epidermis where melanocytes reside. The skin is a complex organ, with a variety of resident cell types. We sought to better define the microenvironment and distinct cellular contributions during autoimmunity in vitiligo, and we found that the epidermis is a chemokine-high niche in both a mouse model and human vitiligo. Analysis of chemokine expression in mouse skin showed that CXCL9 and CXCL10 expression strongly correlate with disease activity, whereas CXCL10 alone correlates with severity, supporting them as potential biomarkers for following disease progression. Further studies in both our mouse model and human patients showed that keratinocytes were the major chemokine producers throughout the course of disease, and functional studies using a conditional signal transducer and activator of transcription (STAT)-1 knockout mouse showed that IFN-γ signaling in keratinocytes was critical for disease progression and proper autoreactive T-cell homing to the epidermis. In contrast, epidermal immune cell populations including endogenous T cells, Langerhans cells, and γδ T cells were not required. These results have important clinical implications, because topical therapies that target IFN-γ signaling in keratinocytes could be safe and effective new treatments, and skin expression of these chemokines could be used to monitor disease activity and treatment responses.",
author = "Richmond, {Jillian M.} and Bangari, {Dinesh S.} and Essien, {Kingsley I.} and Currimbhoy, {Sharif D.} and Groom, {Joanna R.} and Pandya, {Amit G.} and Youd, {Michele E.} and Luster, {Andrew D.} and Harris, {John E.}",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.jid.2016.09.016",
language = "English (US)",
volume = "137",
pages = "350--358",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease

AU - Richmond, Jillian M.

AU - Bangari, Dinesh S.

AU - Essien, Kingsley I.

AU - Currimbhoy, Sharif D.

AU - Groom, Joanna R.

AU - Pandya, Amit G.

AU - Youd, Michele E.

AU - Luster, Andrew D.

AU - Harris, John E.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Vitiligo is an autoimmune disease of the skin that results in the destruction of melanocytes and the clinical appearance of white spots. Disease pathogenesis depends on IFN-γ and IFN-γ–induced chemokines to promote T-cell recruitment to the epidermis where melanocytes reside. The skin is a complex organ, with a variety of resident cell types. We sought to better define the microenvironment and distinct cellular contributions during autoimmunity in vitiligo, and we found that the epidermis is a chemokine-high niche in both a mouse model and human vitiligo. Analysis of chemokine expression in mouse skin showed that CXCL9 and CXCL10 expression strongly correlate with disease activity, whereas CXCL10 alone correlates with severity, supporting them as potential biomarkers for following disease progression. Further studies in both our mouse model and human patients showed that keratinocytes were the major chemokine producers throughout the course of disease, and functional studies using a conditional signal transducer and activator of transcription (STAT)-1 knockout mouse showed that IFN-γ signaling in keratinocytes was critical for disease progression and proper autoreactive T-cell homing to the epidermis. In contrast, epidermal immune cell populations including endogenous T cells, Langerhans cells, and γδ T cells were not required. These results have important clinical implications, because topical therapies that target IFN-γ signaling in keratinocytes could be safe and effective new treatments, and skin expression of these chemokines could be used to monitor disease activity and treatment responses.

AB - Vitiligo is an autoimmune disease of the skin that results in the destruction of melanocytes and the clinical appearance of white spots. Disease pathogenesis depends on IFN-γ and IFN-γ–induced chemokines to promote T-cell recruitment to the epidermis where melanocytes reside. The skin is a complex organ, with a variety of resident cell types. We sought to better define the microenvironment and distinct cellular contributions during autoimmunity in vitiligo, and we found that the epidermis is a chemokine-high niche in both a mouse model and human vitiligo. Analysis of chemokine expression in mouse skin showed that CXCL9 and CXCL10 expression strongly correlate with disease activity, whereas CXCL10 alone correlates with severity, supporting them as potential biomarkers for following disease progression. Further studies in both our mouse model and human patients showed that keratinocytes were the major chemokine producers throughout the course of disease, and functional studies using a conditional signal transducer and activator of transcription (STAT)-1 knockout mouse showed that IFN-γ signaling in keratinocytes was critical for disease progression and proper autoreactive T-cell homing to the epidermis. In contrast, epidermal immune cell populations including endogenous T cells, Langerhans cells, and γδ T cells were not required. These results have important clinical implications, because topical therapies that target IFN-γ signaling in keratinocytes could be safe and effective new treatments, and skin expression of these chemokines could be used to monitor disease activity and treatment responses.

UR - http://www.scopus.com/inward/record.url?scp=85010297625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010297625&partnerID=8YFLogxK

U2 - 10.1016/j.jid.2016.09.016

DO - 10.1016/j.jid.2016.09.016

M3 - Article

C2 - 27686391

AN - SCOPUS:85010297625

VL - 137

SP - 350

EP - 358

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -