Kidney transplant recipients with long incubation-positive antiglobulin-negative T cell crossmatches

C. A. Pettaway, C. Freeman, J. H. Helderman, P. Stastny

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Low-affinity, cold-reactive antibodies easily removed by washing were not detected by the antiglobulin technique but killed T lymphocytes when washing was omitted, incubation was prolonged, and cytotoxic tests were incubated at room temperature or at 4°C. These antibodies were present in approximately 25% of sera from dialysis patients. Only a subset of such sera (22%) reacted with autologous lymphocytes. The majority (86%) appeared to detect non-HLA antigens. A small number (14%) detected class I HLA antigens. Two patients transplanted with antiglobulin-negative, T-warm-negative crossmatch results, but positive cytotoxicity after a 2-hr incubation without washing, rapidly lost their grafts (< 1 month) due to rejection. Their sera contained antibodies against non-HLA alloantigens expressed on lymphocytes and platelets, but not on granulocytes or erythhrocytes. Two other patients with positive autoantibody tests exhibiting similar crossmatches with the current serum were transplated recently. Both of them retain their grafts with good function at one month. In two other cases, the recipients were unreactive against the donor in current serum, but displayed an antiglobulin-negative, 2-hr cytotoxicity-positive pattern in a previously drawn serum specimen. One patient continues to have stable renal function after 10 months. The other patient lost the transplant as a result of renal artery thrombus thought not be be immunologic in origin. Work is continuing to define the specificity and determine the clinicl relevance of such cold-reactive antibodies.

Original languageEnglish (US)
Pages (from-to)529-533
Number of pages5
JournalTransplantation
Volume44
Issue number4
StatePublished - 1987

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Anti-Idiotypic Antibodies
T-Lymphocytes
Kidney
Serum
Antibodies
Transplants
Lymphocytes
Histocompatibility Antigens Class I
Isoantigens
Renal Artery
HLA Antigens
Granulocytes
Autoantibodies
Transplant Recipients
Dialysis
Thrombosis
Blood Platelets
Tissue Donors
Antigens
Temperature

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Pettaway, C. A., Freeman, C., Helderman, J. H., & Stastny, P. (1987). Kidney transplant recipients with long incubation-positive antiglobulin-negative T cell crossmatches. Transplantation, 44(4), 529-533.

Kidney transplant recipients with long incubation-positive antiglobulin-negative T cell crossmatches. / Pettaway, C. A.; Freeman, C.; Helderman, J. H.; Stastny, P.

In: Transplantation, Vol. 44, No. 4, 1987, p. 529-533.

Research output: Contribution to journalArticle

Pettaway, CA, Freeman, C, Helderman, JH & Stastny, P 1987, 'Kidney transplant recipients with long incubation-positive antiglobulin-negative T cell crossmatches', Transplantation, vol. 44, no. 4, pp. 529-533.
Pettaway, C. A. ; Freeman, C. ; Helderman, J. H. ; Stastny, P. / Kidney transplant recipients with long incubation-positive antiglobulin-negative T cell crossmatches. In: Transplantation. 1987 ; Vol. 44, No. 4. pp. 529-533.
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