Kinesin-dependent transport of keratin filaments: A unified mechanism for intermediate filament transport

Amélie Robert, Peirun Tian, Stephen A. Adam, Mark Kittisopikul, Khuloud Jaqaman, Robert D. Goldman, Vladimir I. Gelfand

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Keratin intermediate filaments (IFs) are the major cytoskeletal component in epithelial cells. The dynamics ofkeratinIFshavebeendescribedtodependmostlyonthe actincytoskeleton,but the rapid transport of fully polymerized keratin filaments has not been reported. In this work, we used a combination of photoconversion experiments and clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated protein 9 genome editing to study the role ofmicrotubules and microtubulemotors in keratin filament transport. We found that long keratin filaments, like other types of IFs, are transported along microtubules by kinesin-1. Our data revealed that keratin and vimentin are nonconventional kinesin-1 cargoes because their transport did not require kinesin light chains,which are a typical adapter for kinesin-dependent cargo transport. Furthermore, we found that the same domain of the kinesin heavy chain tail is involved in keratin and vimentin IF transport, strongly suggesting that multiple types of IFs move along microtubules using an identical mechanism.

Original languageEnglish (US)
Pages (from-to)388-399
Number of pages12
JournalFASEB Journal
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Kinesin
Intermediate Filaments
Keratins
Clustered Regularly Interspaced Short Palindromic Repeats
Vimentin
Microtubules
Genes
Epithelial Cells
Light
Proteins

Keywords

  • KIF5B
  • Photoconversion
  • Vimentin

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Robert, A., Tian, P., Adam, S. A., Kittisopikul, M., Jaqaman, K., Goldman, R. D., & Gelfand, V. I. (2019). Kinesin-dependent transport of keratin filaments: A unified mechanism for intermediate filament transport. FASEB Journal, 33(1), 388-399. https://doi.org/10.1096/fj.201800604R

Kinesin-dependent transport of keratin filaments : A unified mechanism for intermediate filament transport. / Robert, Amélie; Tian, Peirun; Adam, Stephen A.; Kittisopikul, Mark; Jaqaman, Khuloud; Goldman, Robert D.; Gelfand, Vladimir I.

In: FASEB Journal, Vol. 33, No. 1, 01.01.2019, p. 388-399.

Research output: Contribution to journalArticle

Robert, A, Tian, P, Adam, SA, Kittisopikul, M, Jaqaman, K, Goldman, RD & Gelfand, VI 2019, 'Kinesin-dependent transport of keratin filaments: A unified mechanism for intermediate filament transport', FASEB Journal, vol. 33, no. 1, pp. 388-399. https://doi.org/10.1096/fj.201800604R
Robert, Amélie ; Tian, Peirun ; Adam, Stephen A. ; Kittisopikul, Mark ; Jaqaman, Khuloud ; Goldman, Robert D. ; Gelfand, Vladimir I. / Kinesin-dependent transport of keratin filaments : A unified mechanism for intermediate filament transport. In: FASEB Journal. 2019 ; Vol. 33, No. 1. pp. 388-399.
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