Kinetic profiles of p300 occupancy in vivo predict common of promoter structure and coactivator recruitment

James L. Smith, Wendy J. Freebern, Irene Collins, Adriana De Siervi, Idalia Montano, Cynthia M. Haggerty, Markey C. McNutt, Wayne G. Butscher, Inna Dzekunovat, David W. Petersen, Ernest Kawasaki, Juanita L. Merchant, Kevin Gardner

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Understanding the language encrypted in the gene regulatory regions of the human genome is a challenging goal for the genomic era. Although customary extrapolations from steady-state mRNA levels have been effective, deciphering these regulatory codes will require additional empirical data sets that more closely reflect the dynamic progression of molecular events responsible for inducible transcription. We describe an approach using chromatin immunoprecipitation to profile the kinetic occupancy of the transcriptional coactivator and histone acetyltransferase p300 at numerous mitogen-induced genes in activated T cells. Comparison of these profiles reveals a class of promoters that share common patterns of inducible expression, p300 recruitment, dependence on selective p300 domains, and sensitivity to histone deacetylase inhibitors. Remarkably, this class also shares an evolutionarily conserved promoter composition and structure that accurately predicts additional human genes with similar functional attributes. This "reverse genomic" approach will have broad application for the genome-wide classification of promoter structure and function.

Original languageEnglish (US)
Pages (from-to)11554-11559
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number32
DOIs
StatePublished - Aug 10 2004

ASJC Scopus subject areas

  • General

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