Kinetics of cyclocreatine and Na+ cotransport in human breast cancer cells: Mechanism of activity

Nimrod Maril, Hadassa Degani, Edna Rushkin, A. Dean Sherry, Mildred Cohn

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The growth-inhibitory effect of cyclocreatine (CCr) and the kinetics of CCr and Na+ cotransport were investigated in MCF7 human breast cancer cells and its adriamycin-resistant subline with use of 31P- and 23Na-NMR spectroscopy. The growth-inhibitory effect in the resistant line occurred at a lower CCr concentration and was more pronounced than in the wild-type line. This correlated with an ~10-fold higher affinity of CCr to the transporter in the resistant line. The passive diffusion coefficient of CCr was also higher in the resistant line by three- to fourfold. The transport of CCr was accompanied by a rapid increase in intracellular Na+. This increase was found to depend on the rate of CCr transport and varied differently with CCr concentration in the two cell lines. It is proposed that the cotransport of CCr and Na+ followed by increased Na+ concentration, together with the accumulation of the highly charged phosphocyclocreatine, are responsible for cell swelling and death.

Original languageEnglish (US)
Pages (from-to)C708-C716
JournalAmerican Journal of Physiology - Cell Physiology
Volume277
Issue number4 46-4
StatePublished - Nov 19 1999

Keywords

  • Cell perfusion
  • Phosphorus-31 nuclear magnetic resonance
  • Sodium-23 nuclear magnetic resonance

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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