Kinetics of glucose delivery to subcutaneous tissue in rats measured with 0.3-mm amperometric microsensors

C. P. Quinn, M. V. Pishko, D. W. Schmidtke, M. Ishikawa, J. G. Wagner, Philip Raskin, J. A. Hubbell, A. Heller

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The time between intravenous injection of a glucose bolus and the time the glucose concentration peaked in the subcutaneous tissue was measured in pentobarbital-anesthetized rats with implanted 290-μm-diameter amperometric sensors. Boluses of 100, 200, and 400 mg/kg body wt were injected. The glucose concentration in the jugular vein was monitored by frequent withdrawal and analysis of samples. The glucose concentration in the subcutaneous tissue was continuously monitored with the sensors. The times required for the subcutaneously implanted sensor to reach its maximum current, corrected for sensor response times, were 7.5 ± 3.9, 9.8 ± 5.5, and 10.0 ± 4.4 min for the smallest to the largest dose, respectively. The shorter delay in response to the smallest dose was statistically significant (P < 0.03). The results were consistent with dilution of the bolus in the cardiovascular system and transport of glucose by both diffusion and facilitated transport via a saturable mediator. An understanding of the differences in the dynamics of venous vs. subcutaneous response to a glucose dose is important in developing algorithms for the control of blood glucose based on a subcutaneous measurement.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume269
Issue number1 32-1
StatePublished - 1995

Fingerprint

Microsensors
Subcutaneous Tissue
Rats
Tissue
kinetics
Glucose
glucose
Kinetics
rats
Sensors
dosage
Facilitated Diffusion
Amperometric sensors
Cardiovascular system
pentobarbital
cardiovascular system
Jugular Veins
jugular vein
Pentobarbital
Cardiovascular System

Keywords

  • electrode
  • facilitated transport
  • intravenous glucose tolerance tests
  • vascular permeability

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Kinetics of glucose delivery to subcutaneous tissue in rats measured with 0.3-mm amperometric microsensors. / Quinn, C. P.; Pishko, M. V.; Schmidtke, D. W.; Ishikawa, M.; Wagner, J. G.; Raskin, Philip; Hubbell, J. A.; Heller, A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 269, No. 1 32-1, 1995.

Research output: Contribution to journalArticle

Quinn, CP, Pishko, MV, Schmidtke, DW, Ishikawa, M, Wagner, JG, Raskin, P, Hubbell, JA & Heller, A 1995, 'Kinetics of glucose delivery to subcutaneous tissue in rats measured with 0.3-mm amperometric microsensors', American Journal of Physiology - Endocrinology and Metabolism, vol. 269, no. 1 32-1.
Quinn, C. P. ; Pishko, M. V. ; Schmidtke, D. W. ; Ishikawa, M. ; Wagner, J. G. ; Raskin, Philip ; Hubbell, J. A. ; Heller, A. / Kinetics of glucose delivery to subcutaneous tissue in rats measured with 0.3-mm amperometric microsensors. In: American Journal of Physiology - Endocrinology and Metabolism. 1995 ; Vol. 269, No. 1 32-1.
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