Kinetochore-associated Stu2 promotes chromosome biorientation in vivo

Matthew P. Miller, Rena K. Evans, Alex Zelter, Elisabeth A. Geyer, Michael J. MacCoss, Luke M. Rice, Trisha N. Davis, Charles L. Asbury, Sue Biggins

Research output: Contribution to journalArticle

Abstract

Accurate segregation of chromosomes to daughter cells is a critical aspect of cell division. It requires the kinetochores on duplicated chromosomes to biorient, attaching to microtubules from opposite poles of the cell. Bioriented attachments come under tension, while incorrect attachments lack tension and must be released to allow proper attachments to form. A well-studied error correction pathway is mediated by the Aurora B kinase, which destabilizes low tension-bearing attachments. We recently discovered that in vitro, kinetochores display an additional intrinsic tension-sensing pathway that utilizes Stu2. The contribution of kinetochore-associated Stu2 to error correction in cells, however, was unknown. Here, we identify a Stu2 mutant that abolishes its kinetochore function and show that it causes biorientation defects in vivo. We also show that this Stu2-mediated pathway functions together with the Aurora B-mediated pathway. Altogether, our work indicates that cells employ multiple pathways to ensure biorientation and the accuracy of chromosome segregation.

Original languageEnglish (US)
Pages (from-to)e1008423
JournalPLoS genetics
Volume15
Issue number10
DOIs
StatePublished - Oct 1 2019

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Kinetochores
kinetochores
chromosome
Chromosomes
chromosomes
Chromosome Segregation
chromosome segregation
error correction
aurora
Aurora Kinase B
cells
Microtubules
Cell Division
microtubules
defect
cell division
phosphotransferases (kinases)
mutants

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Miller, M. P., Evans, R. K., Zelter, A., Geyer, E. A., MacCoss, M. J., Rice, L. M., ... Biggins, S. (2019). Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. PLoS genetics, 15(10), e1008423. https://doi.org/10.1371/journal.pgen.1008423

Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. / Miller, Matthew P.; Evans, Rena K.; Zelter, Alex; Geyer, Elisabeth A.; MacCoss, Michael J.; Rice, Luke M.; Davis, Trisha N.; Asbury, Charles L.; Biggins, Sue.

In: PLoS genetics, Vol. 15, No. 10, 01.10.2019, p. e1008423.

Research output: Contribution to journalArticle

Miller, MP, Evans, RK, Zelter, A, Geyer, EA, MacCoss, MJ, Rice, LM, Davis, TN, Asbury, CL & Biggins, S 2019, 'Kinetochore-associated Stu2 promotes chromosome biorientation in vivo', PLoS genetics, vol. 15, no. 10, pp. e1008423. https://doi.org/10.1371/journal.pgen.1008423
Miller MP, Evans RK, Zelter A, Geyer EA, MacCoss MJ, Rice LM et al. Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. PLoS genetics. 2019 Oct 1;15(10):e1008423. https://doi.org/10.1371/journal.pgen.1008423
Miller, Matthew P. ; Evans, Rena K. ; Zelter, Alex ; Geyer, Elisabeth A. ; MacCoss, Michael J. ; Rice, Luke M. ; Davis, Trisha N. ; Asbury, Charles L. ; Biggins, Sue. / Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. In: PLoS genetics. 2019 ; Vol. 15, No. 10. pp. e1008423.
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