Kinsenoside isolated from anoectochilus formosanus suppresses LPS-stimulated inflammatory reactions in macrophages and endotoxin shock in mice

Hung Bo Hsiao, Jin Bin Wu, Ho Lin, Wen Chuan Lin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In the present study, we reported that kinsenoside, a major component of Anoectochilus formosanus, inhibited inflammatory reactions in mouse peritoneal lavage macrophages and protects mice from endotoxin shock. In LPS-stimulated mouse peritoneal lavage macrophages, kinsenoside inhibited the inflammatory mediators, such as nitric oxide, TNF-α, IL-1β, monocyte chemoattractant protein 1, and macrophage migration inhibitory factor production. Furthermore, kinsenoside decreased the formation of a nuclear factor κB-DNA complex and nuclear p65 and p50 protein levels. Kinsenoside inhibited nuclear factor κB translocation through both IκBα-dependent and -independent pathway. In contrast, it stimulated anti-inflammatory cytokine IL-10 generation and enhanced the mRNA expression of IL-10 and suppressor of cytokine signaling 3 in the same cells induced by LPS. In an animal model, both pretreatment and posttreatment of kinsenoside increased the survival rate of ICR mice challenged by LPS (80 mg/kg, i.p.). Pretreatment with kinsenoside decreased serum levels of TNF-α, IL-1β, IL-10, monocyte chemoattractant protein 1, and migration inhibitory factor at 1 h after sublethal dose of LPS (40 mg/kg, i.p.) in mice. In contrast, kinsenoside enhanced serum IL-10 level at 24 h after LPS injection in mice. In conclusion, kinsenoside inhibited the production of inflammatory mediators and enhanced anti-inflammatory cytokine generation. Therefore, kinsenoside can alleviate acute inflammatory hazards.

Original languageEnglish (US)
Pages (from-to)184-190
Number of pages7
JournalShock
Volume35
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Endotoxins
Shock
Macrophages
Interleukin-10
Peritoneal Lavage
Chemokine CCL2
Peritoneal Macrophages
Cytokines
Interleukin-1
Anti-Inflammatory Agents
B-Form DNA
Macrophage Migration-Inhibitory Factors
3-glucopyranosyloxybutanolide
Inbred ICR Mouse
Serum
Nitric Oxide
Animal Models
Messenger RNA
Injections

Keywords

  • Cytokine
  • inflammation
  • kinsenoside
  • LPS

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine

Cite this

Kinsenoside isolated from anoectochilus formosanus suppresses LPS-stimulated inflammatory reactions in macrophages and endotoxin shock in mice. / Hsiao, Hung Bo; Wu, Jin Bin; Lin, Ho; Lin, Wen Chuan.

In: Shock, Vol. 35, No. 2, 02.2011, p. 184-190.

Research output: Contribution to journalArticle

Hsiao, Hung Bo ; Wu, Jin Bin ; Lin, Ho ; Lin, Wen Chuan. / Kinsenoside isolated from anoectochilus formosanus suppresses LPS-stimulated inflammatory reactions in macrophages and endotoxin shock in mice. In: Shock. 2011 ; Vol. 35, No. 2. pp. 184-190.
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