Abstract
Human anaphylatoxin C3a, derived from complement component C3 has been crystallized and its crystal structure determined at 3.2 A resolution based on multiple isomorphous replacement. The electron density map was interpretable in terms of the known chemical sequence and a molecular model constructed. The molecule has dimensions of 42 x 22 x 16 A. It resembles a drumstick. It is constructed from two helical segments Tyr15 to Met27 and Gly46 to Ser71 connected by a loop. Residues 1 to 14 are flexible. The C-terminal residues are in irregular conformation. The crystal structure analysis establishes the disulphide linkages as Cys22-Cys49, Cys23-Cys56, Cys36-Cys57.
Original language | English (US) |
---|---|
Pages (from-to) | 1389-1400 |
Number of pages | 12 |
Journal | Hoppe-Seyler's Zeitschrift fur Physiologische Chemie |
Volume | 361 |
Issue number | 2 |
DOIs | |
State | Published - 1980 |
Keywords
- Anaphylatoxin
- complement
- crystal structure
ASJC Scopus subject areas
- Biochemistry