Ku is associated with the telomere in mammals

Hsin Ling Hsu, David Gilley, Elizabeth H. Blackburn, David J. Chen

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Telomeres are specialized DNA/protein complexes that comprise the ends of eukaryotic chromosomes. The highly expressed Ku heterodimer, composed of 70 and 80 K(d) subunits (Ku70 and Ku80), is the high-affinity DNA binding component of the DNA-dependent protein kinase. Ku is critical for nonhomologous DNA double-stranded break repair and site-specific recombination of V(D)J gene segments. Ku also plays an important role in telomere maintenance in yeast. Herein, we report, using an in vivo crosslinking method, that human and hamster telomeric DNAs specifically coimmunoprecipitate with human Ku80 after crosslinking. Localization of Ku to the telomere does not depend on the DNA-dependent protein kinase catalytic component. These findings suggest a direct link between Ku and the telomere in mammalian cells.

Original languageEnglish (US)
Pages (from-to)12454-12458
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number22
DOIs
StatePublished - Oct 26 1999

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Telomere
Mammals
DNA-Activated Protein Kinase
DNA
V(D)J Recombination
Double-Stranded DNA Breaks
Cricetinae
Chromosomes
Yeasts
Maintenance
Genes
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Ku is associated with the telomere in mammals. / Hsu, Hsin Ling; Gilley, David; Blackburn, Elizabeth H.; Chen, David J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 22, 26.10.1999, p. 12454-12458.

Research output: Contribution to journalArticle

Hsu, Hsin Ling ; Gilley, David ; Blackburn, Elizabeth H. ; Chen, David J. / Ku is associated with the telomere in mammals. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 22. pp. 12454-12458.
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