L-myc cooperates with ras to transform primary rat embryo fibroblasts.

M. J. Birrer, S. Segal, J. S. DeGreve, F. Kaye, E. A. Sausville, J. D. Minna

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Recent molecular analysis has revealed that L-myc has several domains of extremely conserved amino acid sequence homology with c-myc and N-myc, suggesting similarity of function. We tested the biologic activity of L-myc by using an expression vector containing a cDNA clone coding for the major open reading frame in the 3.9-kilobase mRNA of L-myc under the control of a strong promoter (Moloney long terminal repeat) and found that L-myc complemented an activated ras gene in transforming primary rat embryo fibroblasts. However, the efficiency of transformation was 1 to 10% of that seen with the c-myc and simian virus 40 (SV40) controls. The L-myc/ras transformants initially grew more slowly than c-myc or SV40 transformants, but once established as continuous cell lines, they were indistinguishable from cell lines derived from c-myc/ras or SV40/ras transfectants as determined by morphology, soft-agar cloning, and tumorigenicity in nude mice.

Original languageEnglish (US)
Pages (from-to)2668-2673
Number of pages6
JournalMolecular and cellular biology
Volume8
Issue number6
DOIs
StatePublished - Jun 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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