L1, a novel target of β-catenin signaling, transforms cells and is expressed at the invasive front of colon cancers

Nancy Gavert, Maralice Conacci-Sorrell, Daniela Gast, Annette Schneider, Peter Altevogt, Thomas Brabletz, Avri Ben-Ze'Ev

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

A berrant β-catenin-TCF target gene activation plays a key role in colorectal cancer, both in the initiation stage and during invasion and metastasis. We identified the neuronal cell adhesion molecule L1, as a target gene of β-catenin-TCF signaling in colorectal cancer cells. L1 expression was high in sparse cultures and coregulated with ADAM10, a metalloprotease involved in cleaving and shedding L1's extracellular domain. L1 expression conferred increased cell motility, growth in low serum, transformation and tumorigenesis, whereas its suppression in colon cancer cells decreased motility. L1 was exclusively localized in the invasive front of human colorectal tumors together with ADAM10. The transmembrane localization and shedding of L1 by metalloproteases could be useful for detection and as target for colon cancer therapy.

Original languageEnglish (US)
Pages (from-to)633-642
Number of pages10
JournalJournal of Cell Biology
Volume168
Issue number4
DOIs
StatePublished - Feb 14 2005

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Catenins
Colonic Neoplasms
Colorectal Neoplasms
Metalloproteases
Cell Movement
Neuronal Cell Adhesion Molecules
Neural Cell Adhesion Molecule L1
Transcriptional Activation
Carcinogenesis
Neoplasm Metastasis
Growth
Serum
Genes
Therapeutics

ASJC Scopus subject areas

  • Cell Biology

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L1, a novel target of β-catenin signaling, transforms cells and is expressed at the invasive front of colon cancers. / Gavert, Nancy; Conacci-Sorrell, Maralice; Gast, Daniela; Schneider, Annette; Altevogt, Peter; Brabletz, Thomas; Ben-Ze'Ev, Avri.

In: Journal of Cell Biology, Vol. 168, No. 4, 14.02.2005, p. 633-642.

Research output: Contribution to journalArticle

Gavert, Nancy ; Conacci-Sorrell, Maralice ; Gast, Daniela ; Schneider, Annette ; Altevogt, Peter ; Brabletz, Thomas ; Ben-Ze'Ev, Avri. / L1, a novel target of β-catenin signaling, transforms cells and is expressed at the invasive front of colon cancers. In: Journal of Cell Biology. 2005 ; Vol. 168, No. 4. pp. 633-642.
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