Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients

Baowei Fei, Guolan Lu, Martin T. Halicek, Xu Wang, Hongzheng Zhang, James V. Little, Kelly R. Magliocca, Mihir Patel, Christopher C. Griffith, Mark W. El-Deiry, Amy Y. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Hyperspectral imaging (HSI) is a relatively new modality in medicine and can have many potential applications. In this study, we developed label-free hyperspectral imaging for tumor margin assessment. HSI data, hypercube (x,y,λ), consists of a series of images of the same field of view that are acquired at different wavelengths. Every pixel in the hypercube has an optical spectrum. We collected surgical tissue specimens from 16 human subjects who underwent head and neck (H&N) cancer surgery. We acquired both HSI, autofluorescence images, and fluorescence images with 2-NBDG and proflavine from the specimens. Digitized histologic slides were examined by an H&N pathologist. We developed image preprocessing and classification methods for HSI data and differentiate cancer from benign tissue. The hyperspectral imaging and classification method was able to distinguish between cancer and normal tissue from oral cavity with an average accuracy of 90±8%, sensitivity of 89±9%, and specificity of 91±6%. This study suggests that label-free hyperspectral imaging has great potential for surgical margin assessment in tissue specimens of H&N cancer patients. Further development of the imaging technology and quantification methods is warranted for its application in image-guided surgery.

Original languageEnglish (US)
Title of host publication2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Subtitle of host publicationSmarter Technology for a Healthier World, EMBC 2017 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4041-4045
Number of pages5
ISBN (Electronic)9781509028092
DOIs
StatePublished - Sep 13 2017
Externally publishedYes
Event39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017 - Jeju Island, Korea, Republic of
Duration: Jul 11 2017Jul 15 2017

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017
CountryKorea, Republic of
CityJeju Island
Period7/11/177/15/17

Fingerprint

Labels
Tissue
Optical Imaging
Neoplasms
Proflavine
Computer-Assisted Surgery
Surgery
Mouth
Neck
Bioelectric potentials
Head
Medicine
Hyperspectral imaging
Margins of Excision
Technology
Sensitivity and Specificity
Tumors
Pixels
Fluorescence
Imaging techniques

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Fei, B., Lu, G., Halicek, M. T., Wang, X., Zhang, H., Little, J. V., ... Chen, A. Y. (2017). Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings (pp. 4041-4045). [8037743] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2017.8037743

Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. / Fei, Baowei; Lu, Guolan; Halicek, Martin T.; Wang, Xu; Zhang, Hongzheng; Little, James V.; Magliocca, Kelly R.; Patel, Mihir; Griffith, Christopher C.; El-Deiry, Mark W.; Chen, Amy Y.

2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. p. 4041-4045 8037743 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fei, B, Lu, G, Halicek, MT, Wang, X, Zhang, H, Little, JV, Magliocca, KR, Patel, M, Griffith, CC, El-Deiry, MW & Chen, AY 2017, Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. in 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings., 8037743, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, Institute of Electrical and Electronics Engineers Inc., pp. 4041-4045, 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2017, Jeju Island, Korea, Republic of, 7/11/17. https://doi.org/10.1109/EMBC.2017.8037743
Fei B, Lu G, Halicek MT, Wang X, Zhang H, Little JV et al. Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2017. p. 4041-4045. 8037743. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2017.8037743
Fei, Baowei ; Lu, Guolan ; Halicek, Martin T. ; Wang, Xu ; Zhang, Hongzheng ; Little, James V. ; Magliocca, Kelly R. ; Patel, Mihir ; Griffith, Christopher C. ; El-Deiry, Mark W. ; Chen, Amy Y. / Label-free hyperspectral imaging and quantification methods for surgical margin assessment of tissue specimens of cancer patients. 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Smarter Technology for a Healthier World, EMBC 2017 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 4041-4045 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).
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