Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI

Deborah I Friedman, J. Kelly Johnson, Regina L. Chorsky, Edward G. Stopa

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The carbocyanine dye, DiI, was used to demonstrate human retinohypothalamic tract (RHT) projections in 6 normal human postmortem brains. In 5 of 6 brains, labeling was seen extending from the site of implantation in the distal optic nerve to both the ipsilateral and contralateral suprachiasmatic nuclei. This study confirms the presence of RHT projections in humans, and demonstrates the usefulness of DiI for neuronal tracing in human postmortem tissue.

Original languageEnglish (US)
Pages (from-to)297-302
Number of pages6
JournalBrain Research
Volume560
Issue number1-2
DOIs
StatePublished - Sep 27 1991

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Coloring Agents
Suprachiasmatic Nucleus
Brain
Optic Nerve
3,3'-dihexadecylindocarbocyanine

Keywords

  • Carbocyanine dye
  • Circadian rhythm
  • DiI
  • Hypothalamus
  • Retinohypothalamic tract
  • Suprachiamatic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI. / Friedman, Deborah I; Kelly Johnson, J.; Chorsky, Regina L.; Stopa, Edward G.

In: Brain Research, Vol. 560, No. 1-2, 27.09.1991, p. 297-302.

Research output: Contribution to journalArticle

Friedman, Deborah I ; Kelly Johnson, J. ; Chorsky, Regina L. ; Stopa, Edward G. / Labeling of human retinohypothalamic tract with the carbocyanine dye, DiI. In: Brain Research. 1991 ; Vol. 560, No. 1-2. pp. 297-302.
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