Labeling of the schistosoma mansoni tegument

George Wendt, James J. Collins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Schistosomes are deadly pathogens responsible for the neglected tropical disease schistosomiasis. The parasite’s virulence is aided by a skin-like tissue called the tegument. The study of the tegument is hampered by a lack of tools suitable for visualizing the tissue. Here we describe a novel methodology employing fluorophore-conjugated dextrans that allows specific fluorescent labeling of the tegument and that is compatible with downstream fluorescence-labeling techniques including phalloidin labeling, RNA FISH, and immunofluorescence.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-74
Number of pages10
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2151
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Dextran
  • Fluorescence
  • Fluorescence in situ hybridization
  • Immunofluorescence
  • Phalloidin
  • Schistosome
  • Tegument

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wendt, G., & Collins, J. J. (2020). Labeling of the schistosoma mansoni tegument. In Methods in Molecular Biology (pp. 65-74). (Methods in Molecular Biology; Vol. 2151). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0635-3_6