Lack of aromatase improves cell-mediated immune response after burn

Timothy P. Plackett, Orhan K. Oz, Evan R. Simpson, Elizabeth J. Kovacs

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: Persistent elevation of estrogens after injury or sepsis correlates with increased mortality and a pro-inflammatory state. Given that aromatase is elevated after injury, the enzyme's subsequent conversion of androstenedione and testosterone to estrone and estradiol may be a causative factor for this correlation. Methods: Aromatase knockout (ArKO) and wild type female mice were subjected to a 15% total body surface area burn. The delayed type hypersensitivity (DTH) response and splenocyte production of IL-6 and TNFα were examined 8 days later. Results: Injury in wild type mice is associated with an impairment in the DTH response, as well as with an increase in IL-6 and TNFα production by stimulated splenocytes. However, for ArKO mice, the impairment in DTH was blunted and there was no difference in IL-6 production between sham- and burn-injured mice. Sham-injured ArKO mice produced nearly 50% more TNFα than wild type mice, while injury did not result in a significant increase in TNFα production for ArKO mice. Conclusion: The complete deficiency in aromatase correlated with a decrease in the production of the inflammatory cytokine IL-6 and partial restoration of the DTH response after severe burn. However, a deficiency of aromatase did not effect TNFα production after injury.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalBurns
Volume32
Issue number5
DOIs
StatePublished - Aug 1 2006

Keywords

  • Cell-mediated immunity
  • Estrogen
  • Injury
  • Macrophages
  • Proinflammatory cytokines
  • T lymphocytes

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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