Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane

Aino I. Kinnunen, Raija Sormunen, Harri Elamaa, Lotta Seppinen, R. Tyler Miller, Yoshifumi Ninomiya, Paul A. Janmey, Taina Pihlajaniemi

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Abstract

Collagen XVIII is characterized by three variant N termini, an interrupted collagenous domain, and a C-terminal antiangiogenic domain known as endostatin. We studied here the roles of this collagen type and its variant isoforms in the mouse kidney. Collagen XVIII appeared to be in a polarized orientation in the tubular basement membranes (BMs), the endostatin domain embedded in the BM, and the N terminus residing at the BM-fibrillar matrix interface. In the case of the glomerular BM (GBM), collagen XVIII was expressed in different isoforms depending on the side of the GBM. The orientation appeared polarized here, too, both the endothelial promoter 1-derived short variant of collagen XVIII and the epithelial promoter 2-derived longer variants having their C-terminal endostatin domains embedded in the BM and the N termini at the respective BM-cell interfaces. In addition to loosening of the proximal tubular BM structure, the Col18a1-/- mice showed effacement of the glomerular podocyte foot processes, and microindentation studies showed changes in the mechanical properties of the glomeruli, the Col18a1-/- glomeruli being ∼30% softer than the wild-type. Analysis of promoter-specific knockouts (Col18a1 P1/P1 and Col18a1P2/P2) indicated that tubular BM loosening is due to a lack of the shortest isoform, whereas the glomerular podocyte effacement was due to a lack of the longer isoforms. We suggest that lack of collagen XVIII may also have disparate effects on kidney function in man, but considering the mild physiological findings in the mutant mice, such effects may manifest themselves only late in life or require other compounding molecular changes.

Original languageEnglish (US)
Pages (from-to)7755-7764
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number10
DOIs
StatePublished - Mar 11 2011

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Collagen Type XVIII
Podocytes
Basement Membrane
Protein Isoforms
Kidney
Endostatins
Glomerular Basement Membrane
Membrane structures
Collagen

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane. / Kinnunen, Aino I.; Sormunen, Raija; Elamaa, Harri; Seppinen, Lotta; Miller, R. Tyler; Ninomiya, Yoshifumi; Janmey, Paul A.; Pihlajaniemi, Taina.

In: Journal of Biological Chemistry, Vol. 286, No. 10, 11.03.2011, p. 7755-7764.

Research output: Contribution to journalArticle

Kinnunen, Aino I. ; Sormunen, Raija ; Elamaa, Harri ; Seppinen, Lotta ; Miller, R. Tyler ; Ninomiya, Yoshifumi ; Janmey, Paul A. ; Pihlajaniemi, Taina. / Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 10. pp. 7755-7764.
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abstract = "Collagen XVIII is characterized by three variant N termini, an interrupted collagenous domain, and a C-terminal antiangiogenic domain known as endostatin. We studied here the roles of this collagen type and its variant isoforms in the mouse kidney. Collagen XVIII appeared to be in a polarized orientation in the tubular basement membranes (BMs), the endostatin domain embedded in the BM, and the N terminus residing at the BM-fibrillar matrix interface. In the case of the glomerular BM (GBM), collagen XVIII was expressed in different isoforms depending on the side of the GBM. The orientation appeared polarized here, too, both the endothelial promoter 1-derived short variant of collagen XVIII and the epithelial promoter 2-derived longer variants having their C-terminal endostatin domains embedded in the BM and the N termini at the respective BM-cell interfaces. In addition to loosening of the proximal tubular BM structure, the Col18a1-/- mice showed effacement of the glomerular podocyte foot processes, and microindentation studies showed changes in the mechanical properties of the glomeruli, the Col18a1-/- glomeruli being ∼30{\%} softer than the wild-type. Analysis of promoter-specific knockouts (Col18a1 P1/P1 and Col18a1P2/P2) indicated that tubular BM loosening is due to a lack of the shortest isoform, whereas the glomerular podocyte effacement was due to a lack of the longer isoforms. We suggest that lack of collagen XVIII may also have disparate effects on kidney function in man, but considering the mild physiological findings in the mutant mice, such effects may manifest themselves only late in life or require other compounding molecular changes.",
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AU - Seppinen, Lotta

AU - Miller, R. Tyler

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