Lack of EC-SOD worsens alveolar and vascular development in a neonatal mouse model of bleomycin-induced bronchopulmonary dysplasia and pulmonary hypertension

Cassidy Delaney, Rachel H. Wright, Jen Ruey Tang, Crystal Woods, Leah Villegas, Laurie Sherlock, Rashmin C. Savani, Steven H. Abman, Eva Nozik-Grayck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Pulmonary hypertension (PH) worsens clinical outcomes in former preterm infants with bronchopulmonary dysplasia (BPD). Oxidant stress disrupts alveolar and vascular development in models of BPD. Bleomycin causes oxidative stress and induces BPD and PAH in neonatal rats. Disruption in the vascular endothelial growth factor (VEGF) and nitric oxide signaling pathways contributes to BPD. We hypothesized that loss of EC-SOD would worsen PAH associated with BPD in a neonatal mouse model of bleomycin-induced BPD by disrupting the VEGF/NO signaling pathway.Methods:Neonatal wild-type mice (WT), and mice lacking EC-SOD (EC-SOD KO) received intraperitoneal bleomycin (2 units/kg) or phosphate-buffered saline (PBS) three times weekly and were evaluated at weeks 3 or 4.Results:Lack of EC-SOD impaired alveolar development and resulted in PH (elevated right ventricular systolic pressures, right ventricular hypertrophy (RVH)), decreased vessel density, and increased small vessel muscularization. Exposure to bleomycin further impaired alveolar development, worsened RVH and vascular remodeling. Lack of EC-SOD and bleomycin treatment decreased lung total and phosphorylated VEGFR2 and eNOS protein expression.Conclusion:EC-SOD is critical in preserving normal lung development and loss of EC-SOD results in disrupted alveolar development, PAH and vascular remodeling at baseline, which is further worsened with bleomycin and associated with decreased activation of VEGFR2.

Original languageEnglish (US)
Pages (from-to)634-640
Number of pages7
JournalPediatric Research
Volume78
Issue number6
DOIs
StatePublished - Dec 1 2015

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Bronchopulmonary Dysplasia
Bleomycin
Pulmonary Hypertension
Blood Vessels
Right Ventricular Hypertrophy
Vascular Endothelial Growth Factor A
Lung
Ventricular Remodeling
Ventricular Pressure
Oxidants
Premature Infants
Nitric Oxide
Oxidative Stress
Phosphates
Blood Pressure
Proteins

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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Lack of EC-SOD worsens alveolar and vascular development in a neonatal mouse model of bleomycin-induced bronchopulmonary dysplasia and pulmonary hypertension. / Delaney, Cassidy; Wright, Rachel H.; Tang, Jen Ruey; Woods, Crystal; Villegas, Leah; Sherlock, Laurie; Savani, Rashmin C.; Abman, Steven H.; Nozik-Grayck, Eva.

In: Pediatric Research, Vol. 78, No. 6, 01.12.2015, p. 634-640.

Research output: Contribution to journalArticle

Delaney, Cassidy ; Wright, Rachel H. ; Tang, Jen Ruey ; Woods, Crystal ; Villegas, Leah ; Sherlock, Laurie ; Savani, Rashmin C. ; Abman, Steven H. ; Nozik-Grayck, Eva. / Lack of EC-SOD worsens alveolar and vascular development in a neonatal mouse model of bleomycin-induced bronchopulmonary dysplasia and pulmonary hypertension. In: Pediatric Research. 2015 ; Vol. 78, No. 6. pp. 634-640.
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abstract = "Pulmonary hypertension (PH) worsens clinical outcomes in former preterm infants with bronchopulmonary dysplasia (BPD). Oxidant stress disrupts alveolar and vascular development in models of BPD. Bleomycin causes oxidative stress and induces BPD and PAH in neonatal rats. Disruption in the vascular endothelial growth factor (VEGF) and nitric oxide signaling pathways contributes to BPD. We hypothesized that loss of EC-SOD would worsen PAH associated with BPD in a neonatal mouse model of bleomycin-induced BPD by disrupting the VEGF/NO signaling pathway.Methods:Neonatal wild-type mice (WT), and mice lacking EC-SOD (EC-SOD KO) received intraperitoneal bleomycin (2 units/kg) or phosphate-buffered saline (PBS) three times weekly and were evaluated at weeks 3 or 4.Results:Lack of EC-SOD impaired alveolar development and resulted in PH (elevated right ventricular systolic pressures, right ventricular hypertrophy (RVH)), decreased vessel density, and increased small vessel muscularization. Exposure to bleomycin further impaired alveolar development, worsened RVH and vascular remodeling. Lack of EC-SOD and bleomycin treatment decreased lung total and phosphorylated VEGFR2 and eNOS protein expression.Conclusion:EC-SOD is critical in preserving normal lung development and loss of EC-SOD results in disrupted alveolar development, PAH and vascular remodeling at baseline, which is further worsened with bleomycin and associated with decreased activation of VEGFR2.",
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AU - Villegas, Leah

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