Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: A 13C NMR study

V. Mezzarobba, G. Bielicki, F. M H Jeffrey, M. Mignon, J. P. Renou, J. Grizard, D. Meynial-Denis

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Acetate oxidation was examined by 13C nuclear magnetic resonance in skeletal muscle from adult and old rats. Rats fasted for 5 days were perfused with [2-13C]acetate over 2 h, and muscle extracts were analyzed for [13C]glutamate isotopomers. This study shows that approximately 80 % of acetyl-coenzyme A entering the tricarboxylic cycle was derived from substrate infusion in both adult and old rats, and that the flux through anaplerotic pathways was approximately 21% of the flux through citrate synthase. These data demonstrate that skeletal muscle from adult and old rats oxidizes the same proportion of exogenous acetate.

Original languageEnglish (US)
Pages (from-to)995-1001
Number of pages7
JournalJournal of Experimental Biology
Volume203
Issue number6
StatePublished - Mar 1 2000

Keywords

  • Acetate
  • Ageing
  • C NMR
  • Citric acid cycle
  • Glutamate
  • Rat
  • Skeletal muscle

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

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