Langerhans cell interaction with epidermal y& t cells

K. Yokota, K. Ariizumi, T. Kiaiima, P. R. Berestresser, Takashima

Research output: Contribution to journalArticle

Abstract

Two leukocyte populations reside in murine epidermis: a) Langerhans cells (LC), Ag presenting cells of dendritic cell lineage, and b) dendritic epidermal T cells (DETC), tissue-resident y& T cells. Despite their close approximation in vivo, the extent to which LC and DETC affect each other's function is not known. To address this question, we employed the long-term DC line XS52 and the yS T cell line 7-17, both of which were established from mouse epidermis and both of which retain important features of resident LC and DETC. The XS52 cells, which proliferate maximally in response to added GM-CSF or CSF-1, exhibited an equivalent level of proliferation when cocultured with -irradiated 7-17 DETC. Supernatants collected from anti-CD3 stimulated 7-17 DETC cultures, but not from unstimulated cells, also promoted the growth of XS52 cells, suggesting that one or more soluble factors play roles. This supernatant effect was blocked substantially with antibodies against GM-CSF or the receptor for CSF-1, documenting roles for both GM-CSF and CSF-1. Because the 7-17 DETC secreted these cytokines only after stimulation, our observations are most consistent with the hypothesis that XS52 DC deliver activation signals to DETC for the secretion of GM-CSF and CSF-1, which, in turn, promote the growth of XS52 DC. We propose that LC and DETC interact with each other in situ, thereby regulating their residence and function within the epidermal microenvironment.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

Langerhans cells
T-cells
Langerhans Cells
Cell Communication
T-lymphocytes
T-Lymphocytes
Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
cells
epidermis (animal)
Epidermis
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor Receptors
mice
Cell Lineage
dendritic cells
Growth
Cell culture
Dendritic Cells
cell growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Yokota, K., Ariizumi, K., Kiaiima, T., Berestresser, P. R., & Takashima (1996). Langerhans cell interaction with epidermal y& t cells. FASEB Journal, 10(6).

Langerhans cell interaction with epidermal y& t cells. / Yokota, K.; Ariizumi, K.; Kiaiima, T.; Berestresser, P. R.; Takashima.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Yokota, K, Ariizumi, K, Kiaiima, T, Berestresser, PR & Takashima 1996, 'Langerhans cell interaction with epidermal y& t cells', FASEB Journal, vol. 10, no. 6.
Yokota K, Ariizumi K, Kiaiima T, Berestresser PR, Takashima. Langerhans cell interaction with epidermal y& t cells. FASEB Journal. 1996;10(6).
Yokota, K. ; Ariizumi, K. ; Kiaiima, T. ; Berestresser, P. R. ; Takashima. / Langerhans cell interaction with epidermal y& t cells. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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