Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries

Charles R. Rosenfeld, R. Ann Word, Kevin DeSpain, Xiao Tie Liu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Large conductance K+ channels (BKCa) are expressed in uterine artery (UA) smooth muscle from nonpregnant and pregnant sheep and contribute to the regulation of basal vascular tone and responses to estrogen and vasoconstrictors. To determine if BKCa are expressed in women and contribute to UA function, we collected UA from nonpregnant women (n = 31) at elective hysterectomy and analyzed for subunit protein, localization with immunohistochemistry, and function using endothelium-denuded rings. UA expresses BKCa α -, β1- and β2-subunit protein. KCl and phenylephrine (PE, an α1-agonist) caused dose-dependent vasoconstriction (P <.001), and UA precontracted with PE dose-dependently relaxed with sodium nitroprusside (SNP; P <.001).Tetraethylammonium chloride (TEA, 0.2-1.0 mM), a BKCa inhibitor, dose-dependently increased resting tone (P =.004; 28% ± 5.3% with 1.0 mM), enhanced PE-induced (10-6 M) vasoconstriction (P < .04), and attenuated SNP-induced relaxation at 1.0 mM (P =.02). BKCa are expressed in human UA and modulate vascular function by attenuating vasoconstrictor responses and contributing to nitric oxide-induced vasorelaxation.

Original languageEnglish (US)
Pages (from-to)651-660
Number of pages10
JournalReproductive Sciences
Volume15
Issue number7
DOIs
StatePublished - Sep 2008

Fingerprint

Calcium-Activated Potassium Channels
Uterine Artery
Blood Vessels
Protein Subunits
Vasoconstrictor Agents
Vasoconstriction
Single Nucleotide Polymorphism
Tetraethylammonium
Nitroprusside
Phenylephrine
Hysterectomy
Vasodilation
Endothelium
Smooth Muscle
Sheep
Nitric Oxide
Estrogens
Immunohistochemistry

Keywords

  • α-agonists
  • β1/β2- regulatory subunits
  • Basal tone
  • Myometrium
  • Nitric oxide

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Medicine(all)

Cite this

Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries. / Rosenfeld, Charles R.; Word, R. Ann; DeSpain, Kevin; Liu, Xiao Tie.

In: Reproductive Sciences, Vol. 15, No. 7, 09.2008, p. 651-660.

Research output: Contribution to journalArticle

@article{fcc61dd54f534d36b5cf1b68be787ead,
title = "Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries",
abstract = "Large conductance K+ channels (BKCa) are expressed in uterine artery (UA) smooth muscle from nonpregnant and pregnant sheep and contribute to the regulation of basal vascular tone and responses to estrogen and vasoconstrictors. To determine if BKCa are expressed in women and contribute to UA function, we collected UA from nonpregnant women (n = 31) at elective hysterectomy and analyzed for subunit protein, localization with immunohistochemistry, and function using endothelium-denuded rings. UA expresses BKCa α -, β1- and β2-subunit protein. KCl and phenylephrine (PE, an α1-agonist) caused dose-dependent vasoconstriction (P <.001), and UA precontracted with PE dose-dependently relaxed with sodium nitroprusside (SNP; P <.001).Tetraethylammonium chloride (TEA, 0.2-1.0 mM), a BKCa inhibitor, dose-dependently increased resting tone (P =.004; 28{\%} ± 5.3{\%} with 1.0 mM), enhanced PE-induced (10-6 M) vasoconstriction (P < .04), and attenuated SNP-induced relaxation at 1.0 mM (P =.02). BKCa are expressed in human UA and modulate vascular function by attenuating vasoconstrictor responses and contributing to nitric oxide-induced vasorelaxation.",
keywords = "α-agonists, β1/β2- regulatory subunits, Basal tone, Myometrium, Nitric oxide",
author = "Rosenfeld, {Charles R.} and Word, {R. Ann} and Kevin DeSpain and Liu, {Xiao Tie}",
year = "2008",
month = "9",
doi = "10.1177/1933719108319160",
language = "English (US)",
volume = "15",
pages = "651--660",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "7",

}

TY - JOUR

T1 - Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries

AU - Rosenfeld, Charles R.

AU - Word, R. Ann

AU - DeSpain, Kevin

AU - Liu, Xiao Tie

PY - 2008/9

Y1 - 2008/9

N2 - Large conductance K+ channels (BKCa) are expressed in uterine artery (UA) smooth muscle from nonpregnant and pregnant sheep and contribute to the regulation of basal vascular tone and responses to estrogen and vasoconstrictors. To determine if BKCa are expressed in women and contribute to UA function, we collected UA from nonpregnant women (n = 31) at elective hysterectomy and analyzed for subunit protein, localization with immunohistochemistry, and function using endothelium-denuded rings. UA expresses BKCa α -, β1- and β2-subunit protein. KCl and phenylephrine (PE, an α1-agonist) caused dose-dependent vasoconstriction (P <.001), and UA precontracted with PE dose-dependently relaxed with sodium nitroprusside (SNP; P <.001).Tetraethylammonium chloride (TEA, 0.2-1.0 mM), a BKCa inhibitor, dose-dependently increased resting tone (P =.004; 28% ± 5.3% with 1.0 mM), enhanced PE-induced (10-6 M) vasoconstriction (P < .04), and attenuated SNP-induced relaxation at 1.0 mM (P =.02). BKCa are expressed in human UA and modulate vascular function by attenuating vasoconstrictor responses and contributing to nitric oxide-induced vasorelaxation.

AB - Large conductance K+ channels (BKCa) are expressed in uterine artery (UA) smooth muscle from nonpregnant and pregnant sheep and contribute to the regulation of basal vascular tone and responses to estrogen and vasoconstrictors. To determine if BKCa are expressed in women and contribute to UA function, we collected UA from nonpregnant women (n = 31) at elective hysterectomy and analyzed for subunit protein, localization with immunohistochemistry, and function using endothelium-denuded rings. UA expresses BKCa α -, β1- and β2-subunit protein. KCl and phenylephrine (PE, an α1-agonist) caused dose-dependent vasoconstriction (P <.001), and UA precontracted with PE dose-dependently relaxed with sodium nitroprusside (SNP; P <.001).Tetraethylammonium chloride (TEA, 0.2-1.0 mM), a BKCa inhibitor, dose-dependently increased resting tone (P =.004; 28% ± 5.3% with 1.0 mM), enhanced PE-induced (10-6 M) vasoconstriction (P < .04), and attenuated SNP-induced relaxation at 1.0 mM (P =.02). BKCa are expressed in human UA and modulate vascular function by attenuating vasoconstrictor responses and contributing to nitric oxide-induced vasorelaxation.

KW - α-agonists

KW - β1/β2- regulatory subunits

KW - Basal tone

KW - Myometrium

KW - Nitric oxide

UR - http://www.scopus.com/inward/record.url?scp=52449128966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52449128966&partnerID=8YFLogxK

U2 - 10.1177/1933719108319160

DO - 10.1177/1933719108319160

M3 - Article

VL - 15

SP - 651

EP - 660

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 7

ER -