Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable

Carolyn Katovich Hurley, M. Maiers, J. Ng, D. Wagage, J. Hegland, J. Baisch, R. Endres, M. Fernandez-Vina, U. Heine, S. Hsu, M. Kamoun, Y. Mitsuishi, D. Monos, H. Noreen, L. Perlee, S. Rodriguez-Marino, A. Smith, P. Stastny, M. Trucco, S. Y. YangN. Yu, R. Holsten, R. J. Hartzman, M. Setterholm

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program® unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalTissue Antigens
Volume55
Issue number4
DOIs
StatePublished - 2000

Fingerprint

HLA-A Antigens
HLA-B Antigens
DNA Fingerprinting
Quality Control
Tissue Donors
Unrelated Donors
DNA Primers
Oligonucleotide Probes
Registries
Bone Marrow
Alleles

Keywords

  • Bone marrow registry
  • HLA-A
  • HLA-B
  • Polymerase chain reaction
  • Sequence-specific oligonucleotide typing
  • Sequence-specific primer typing

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Hurley, C. K., Maiers, M., Ng, J., Wagage, D., Hegland, J., Baisch, J., ... Setterholm, M. (2000). Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable. Tissue Antigens, 55(4), 352-358. https://doi.org/10.1034/j.1399-0039.2000.550409.x

Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable. / Hurley, Carolyn Katovich; Maiers, M.; Ng, J.; Wagage, D.; Hegland, J.; Baisch, J.; Endres, R.; Fernandez-Vina, M.; Heine, U.; Hsu, S.; Kamoun, M.; Mitsuishi, Y.; Monos, D.; Noreen, H.; Perlee, L.; Rodriguez-Marino, S.; Smith, A.; Stastny, P.; Trucco, M.; Yang, S. Y.; Yu, N.; Holsten, R.; Hartzman, R. J.; Setterholm, M.

In: Tissue Antigens, Vol. 55, No. 4, 2000, p. 352-358.

Research output: Contribution to journalArticle

Hurley, CK, Maiers, M, Ng, J, Wagage, D, Hegland, J, Baisch, J, Endres, R, Fernandez-Vina, M, Heine, U, Hsu, S, Kamoun, M, Mitsuishi, Y, Monos, D, Noreen, H, Perlee, L, Rodriguez-Marino, S, Smith, A, Stastny, P, Trucco, M, Yang, SY, Yu, N, Holsten, R, Hartzman, RJ & Setterholm, M 2000, 'Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable', Tissue Antigens, vol. 55, no. 4, pp. 352-358. https://doi.org/10.1034/j.1399-0039.2000.550409.x
Hurley, Carolyn Katovich ; Maiers, M. ; Ng, J. ; Wagage, D. ; Hegland, J. ; Baisch, J. ; Endres, R. ; Fernandez-Vina, M. ; Heine, U. ; Hsu, S. ; Kamoun, M. ; Mitsuishi, Y. ; Monos, D. ; Noreen, H. ; Perlee, L. ; Rodriguez-Marino, S. ; Smith, A. ; Stastny, P. ; Trucco, M. ; Yang, S. Y. ; Yu, N. ; Holsten, R. ; Hartzman, R. J. ; Setterholm, M. / Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable. In: Tissue Antigens. 2000 ; Vol. 55, No. 4. pp. 352-358.
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