Large scale enucleation of mammalian cells

G. Veomett; J. Shay; P. V C Hough; D. M. Prescott

Large scale enucleation of mammalian cells

G. Veomett, J. Shay, P. V C Hough, D. M. Prescott

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm² tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.

Original language	English (US)
Title of host publication	Methods in Cell Biology
Pages	1-6
Number of pages	6
Volume	Vol. 13
State	Published - 1976

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Large scale enucleation of mammalian cells",

abstract = "A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm2 tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.",

author = "G. Veomett and J. Shay and Hough, {P. V C} and Prescott, {D. M.}",

year = "1976",

language = "English (US)",

volume = "Vol. 13",

pages = "1--6",

booktitle = "Methods in Cell Biology",

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TY - CHAP

T1 - Large scale enucleation of mammalian cells

AU - Veomett, G.

AU - Shay, J.

AU - Hough, P. V C

AU - Prescott, D. M.

PY - 1976

Y1 - 1976

N2 - A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm2 tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.

AB - A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm2 tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.

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M3 - Chapter

C2 - 1263848

VL - Vol. 13

SP - 1

EP - 6

BT - Methods in Cell Biology

ER -

Large scale enucleation of mammalian cells

Abstract

ASJC Scopus subject areas

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