A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm2 tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.
|Original language||English (US)|
|Title of host publication||Methods in Cell Biology|
|Number of pages||6|
|State||Published - 1976|
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