Large scale enucleation of mammalian cells

G. Veomett, J. Shay, P. V C Hough, D. M. Prescott

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A relatively simple method for obtaining large numbers of enucleated cells and karyoplasts is described. Use is made of 25 cm2 tissue culture flasks both as substrate for cellular growth and as centrifuge tubes. The method is described for mouse L cells, but should be applicable to any cell that grows in monolayers. The L cells were grown in Ham's F12 medium supplemented with 10% fetal bovine serum and antibiotics. The medium for enucleation consists of growth medium supplemented with 10 μg/ml of cytochalasin B.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
Pages1-6
Number of pages6
VolumeVol. 13
StatePublished - 1976

ASJC Scopus subject areas

  • Medicine(all)

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    Veomett, G., Shay, J., Hough, P. V. C., & Prescott, D. M. (1976). Large scale enucleation of mammalian cells. In Methods in Cell Biology (Vol. Vol. 13, pp. 1-6)