TY - JOUR
T1 - Laser microdissection
T2 - Exploring host-bacterial encounters at the front lines
AU - Hooper, Lora V.
N1 - Funding Information:
I thank my father, graphic artist Charles Hooper, for help in creating the figures in this article. I also gratefully acknowledge support from the Burroughs Wellcome Foundation (Career Award in the Biomedical Sciences), and from the Crohn’s and Colitis Foundation of America.
PY - 2004/6
Y1 - 2004/6
N2 - The mucosal surfaces of tissues such as the stomach and intestines are in constant contact with indigenous bacterial populations and are major portals of entry for bacterial pathogens. Host responses to bacterial encounters at these surfaces frequently involve complex interactions between epithelial cells and immune cells, and are thus difficult to model in vitro. Laser microdissection is a technique in which pure populations of host cells are acquired from sections of complex tissue. When coupled with an expanding repertoire of techniques for molecular analysis of microdissected cells, laser microdissection allows host cellular responses to bacteria to be studied in their native tissue context. This approach has already yielded key insights into the nature of mucosal responses to commensal, as well as pathogenic bacteria, and promises to be an important addition to the cellular microbiologist's toolkit.
AB - The mucosal surfaces of tissues such as the stomach and intestines are in constant contact with indigenous bacterial populations and are major portals of entry for bacterial pathogens. Host responses to bacterial encounters at these surfaces frequently involve complex interactions between epithelial cells and immune cells, and are thus difficult to model in vitro. Laser microdissection is a technique in which pure populations of host cells are acquired from sections of complex tissue. When coupled with an expanding repertoire of techniques for molecular analysis of microdissected cells, laser microdissection allows host cellular responses to bacteria to be studied in their native tissue context. This approach has already yielded key insights into the nature of mucosal responses to commensal, as well as pathogenic bacteria, and promises to be an important addition to the cellular microbiologist's toolkit.
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U2 - 10.1016/j.mib.2004.04.004
DO - 10.1016/j.mib.2004.04.004
M3 - Review article
C2 - 15196498
AN - SCOPUS:2942560681
SN - 1369-5274
VL - 7
SP - 290
EP - 295
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
IS - 3
ER -