Laser microdissection

Exploring host-bacterial encounters at the front lines

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The mucosal surfaces of tissues such as the stomach and intestines are in constant contact with indigenous bacterial populations and are major portals of entry for bacterial pathogens. Host responses to bacterial encounters at these surfaces frequently involve complex interactions between epithelial cells and immune cells, and are thus difficult to model in vitro. Laser microdissection is a technique in which pure populations of host cells are acquired from sections of complex tissue. When coupled with an expanding repertoire of techniques for molecular analysis of microdissected cells, laser microdissection allows host cellular responses to bacteria to be studied in their native tissue context. This approach has already yielded key insights into the nature of mucosal responses to commensal, as well as pathogenic bacteria, and promises to be an important addition to the cellular microbiologist's toolkit.

Original languageEnglish (US)
Pages (from-to)290-295
Number of pages6
JournalCurrent Opinion in Microbiology
Volume7
Issue number3
DOIs
StatePublished - Jun 2004

Fingerprint

Microdissection
Lasers
Bacteria
Population Groups
Intestines
Stomach
Mucous Membrane
Epithelial Cells
Population

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology

Cite this

Laser microdissection : Exploring host-bacterial encounters at the front lines. / Hooper, Lora V.

In: Current Opinion in Microbiology, Vol. 7, No. 3, 06.2004, p. 290-295.

Research output: Contribution to journalArticle

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