Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene

Mingxu Xu, Wenling Han, Mingping Qian, Xiaotu Ma, Peiguo Ding, Ying Wang, Donglan Xia, Min Rui, Lu Wang, Yinmei Zhang, Dalong Ma

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The genes for chemokine-like factor (CKLF) and four chemokine-like factor super family members (CKLFSF1-4) are tightly linked on chromosome 16, with only 325bp separating CKLF and CKLFSF1. We used Northern blotting and RT-PCR to show that these two genes are expressed independently of one another. We then used a novel computational promoter prediction method based on the interaction among transcription factor binding sites (TFBSs) to identify a putative promoter region for the CKLFSF1 gene. Our method predicted a promoter region in the last intron of the upstream gene, CKLF. We PCR amplified the predicted promoter region and used a luciferase assay to show that the region was able to drive the luciferase gene. DNA decoy experiments indicated that 214bp fragment neighboring the TATA box markedly inhibited CKLFSF1 gene expression. Sequence analysis of the region revealed a typical TATA box (TATATAA) and multiple potential transcription factor binding sites, providing further evidence for this being a functional promoter for CKLFSF1. This work provides the first evidence of a promoter from one gene located in an intron of another.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume313
Issue number1
DOIs
StatePublished - Jan 2 2004

Keywords

  • Chemokine-like factor
  • Chemokine-like factor super family
  • Intron
  • Luciferase assay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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