Latent transformed growth-inhibiting factor in human malignant effusions

D. K. Podolsky, D. K. Pleskow, H. Jafari

Research output: Contribution to journalArticle

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Abstract

An inhibitor of soft agar colony formation by a human breast carcinoma-derived cell line was found to be present in latent form in the majority of cytology-positive human malignant effusions. Prior to dialysis, addition of human malignant effusions resulted in <10% alteration in efficiency of colony formation by the BT-20 human breast carcinoma cell line (mean efficiency 1 colony/4.3 cells plated at 14 days; mean colony diameter >0.8 mm). After dialysis (membrane cutoff of 3500 molecular weight), 58 of 70 malignant effusions from patients with a variety of epithelial cell carcinomas resulted in 71% mean inhibition of colony formation (range 19.1-98% inhibition). Similar inhibition of anchorage-independent growth was observed for a human colon cancer-derived cell line (HCT-15) but not for polyoma and murine sarcoma virus-transformed rodent fibroblast lines. The malignant effusion-related transformed cell growth-inhibiting factor (TGIF) was sensitive to heat, sulfhydryl reduction, and protease treatment. TGIF-containing effusion resulted in parallel inhibition of thymidine incorporation in sensitive cell types in vitro. TGIF was precipitable in 28-34% ammonium sulfate with reconstitution of activity after resolubilization. TGIF was partially purified by chromatography on Biogel A-0.5 and Biogel P-100 which yielded two peaks of inhibitory activity. The predominant species had an approximate molecular weight of 110,000 and could be recovered as a single species from DEAE-cellulose at relatively high salt concentrations (0.4 M NaCl). A smaller amount of inhibitory activity was recovered from Biogel P-100 or Biogel P-60 with an apparent molecular weight of 55,000. The higher molecular-weight TGIF which appears to be a dimer of the M(r) 55,000 protein is distinguishable from previously described growth-promoting and -inhibiting factors.

Original languageEnglish (US)
Pages (from-to)418-424
Number of pages7
JournalCancer Research
Volume48
Issue number2
StatePublished - 1988

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Intercellular Signaling Peptides and Proteins
Molecular Weight
Dialysis
Murine Sarcoma Viruses
Cell Line
DEAE-Cellulose
Ammonium Sulfate
Growth
Colonic Neoplasms
Thymidine
Agar
Cell Biology
Chromatography
Rodentia
Peptide Hydrolases
Fibroblasts
Salts
Hot Temperature
Epithelial Cells
Breast Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Latent transformed growth-inhibiting factor in human malignant effusions. / Podolsky, D. K.; Pleskow, D. K.; Jafari, H.

In: Cancer Research, Vol. 48, No. 2, 1988, p. 418-424.

Research output: Contribution to journalArticle

Podolsky, DK, Pleskow, DK & Jafari, H 1988, 'Latent transformed growth-inhibiting factor in human malignant effusions', Cancer Research, vol. 48, no. 2, pp. 418-424.
Podolsky, D. K. ; Pleskow, D. K. ; Jafari, H. / Latent transformed growth-inhibiting factor in human malignant effusions. In: Cancer Research. 1988 ; Vol. 48, No. 2. pp. 418-424.
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