TY - JOUR
T1 - LC‐QTOF‐MS and1H NMR metabolomics verifies potential use of greater omentum for Klebsiella pneumoniae biofilm eradication in rats
AU - Teul, Joanna
AU - Deja, Stanisław
AU - Celińska‐janowicz, Katarzyna
AU - Ząbek, Adam
AU - Młynarz, Piotr
AU - Barć, Piotr
AU - Junka, Adam
AU - Smutnicka, Danuta
AU - Bartoszewicz, Marzenna
AU - Pałka, Jerzy
AU - Miltyk, Wojciech
N1 - Funding Information:
This research was founded by the National Science Centre in Poland, grant no. DEC‐ 2012/07/N/NZ4/02528.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/5
Y1 - 2020/5
N2 - Bacterial wound infections are a common problem associated with surgical interventions. In particular, biofilm‐forming bacteria are hard to eradicate, and alternative methods of treatment based on covering wounds with vascularized flaps of tissue are being developed. The greater omentum is a complex organ covering the intestines in the abdomen, which support wound recovery following surgical procedures and exhibit natural antimicrobial activity that could improve biofilm eradication. We investigated changes in rats’ metabolome following Klebsiella pneumoniae infections, as well as the greater omentum’s ability for Klebsiella pneumoniae biofilm eradication. Rats received either sterile implants or implants covered with Klebsiella pneumoniae biofilm (placed in the peritoneum or greater omentum). Metabolic profiles were monitored at days 0, 2, and 5 after surgery using combined proton nuclear magnetic resonance (1H NMR) and high performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry (LC– QTOF‐MS) measurements of urine samples followed by chemometric analysis. Obtained results indicated that grafting of the sterile implant to the greater omentum did not cause major disturbances in rats’ metabolism, whereas the sterile implant located in the peritoneum triggered metabolic perturbations related to tricarboxylic acid (TCA) cycle, as well as choline, tryptophan, and hippurate metabolism. Presence of implants colonized with Klebsiella pneumoniae biofilm resulted in similar levels of metabolic perturbations in both locations. Our findings confirmed that surgical procedures utilizing the greater omentum may have a practical use in wound healing and tissue regeneration in the future.
AB - Bacterial wound infections are a common problem associated with surgical interventions. In particular, biofilm‐forming bacteria are hard to eradicate, and alternative methods of treatment based on covering wounds with vascularized flaps of tissue are being developed. The greater omentum is a complex organ covering the intestines in the abdomen, which support wound recovery following surgical procedures and exhibit natural antimicrobial activity that could improve biofilm eradication. We investigated changes in rats’ metabolome following Klebsiella pneumoniae infections, as well as the greater omentum’s ability for Klebsiella pneumoniae biofilm eradication. Rats received either sterile implants or implants covered with Klebsiella pneumoniae biofilm (placed in the peritoneum or greater omentum). Metabolic profiles were monitored at days 0, 2, and 5 after surgery using combined proton nuclear magnetic resonance (1H NMR) and high performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry (LC– QTOF‐MS) measurements of urine samples followed by chemometric analysis. Obtained results indicated that grafting of the sterile implant to the greater omentum did not cause major disturbances in rats’ metabolism, whereas the sterile implant located in the peritoneum triggered metabolic perturbations related to tricarboxylic acid (TCA) cycle, as well as choline, tryptophan, and hippurate metabolism. Presence of implants colonized with Klebsiella pneumoniae biofilm resulted in similar levels of metabolic perturbations in both locations. Our findings confirmed that surgical procedures utilizing the greater omentum may have a practical use in wound healing and tissue regeneration in the future.
KW - Implants
KW - Klebsiella pneumoniae
KW - Metabolomics
KW - Microbial biofilm eradication
KW - Surgery
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U2 - 10.3390/pathogens9050399
DO - 10.3390/pathogens9050399
M3 - Article
C2 - 32455691
AN - SCOPUS:85085331045
SN - 2076-0817
VL - 9
JO - Pathogens
JF - Pathogens
IS - 5
M1 - 399
ER -