Lens regeneration using endogenous stem cells with gain of visual function

Haotian Lin; Hong Ouyang; Jie Zhu; Shan Huang; Zhenzhen Liu; Shuyi Chen; Guiqun Cao; Gen Li; Robert A J Signer; Yanxin Xu; Christopher Chung; Ying Zhang; Danni Lin; Sherrina Patel; Frances Wu; Huimin Cai; Jiayi Hou; Cindy Wen; Maryam Jafari; Xialin Liu; Lixia Luo; Jin Zhu; Austin Qiu; Rui Hou; Baoxin Chen; Jiangna Chen; David Granet; Christopher Heichel; Fu Shang; Xuri Li; Michal Krawczyk; Dorota Skowronska-Krawczyk; Yujuan Wang; William Shi; Daniel Chen; Zheng Zhong; Sheng Zhong; Liangfang Zhang; Shaochen Chen; Sean J. Morrison; Richard L. Maas; Kang Zhang; Yizhi Liu

doi:10.1038/nature17181

Lens regeneration using endogenous stem cells with gain of visual function

Haotian Lin, Hong Ouyang, Jie Zhu, Shan Huang, Zhenzhen Liu, Shuyi Chen, Guiqun Cao, Gen Li, Robert A J Signer, Yanxin Xu, Christopher Chung, Ying Zhang, Danni Lin, Sherrina Patel, Frances Wu, Huimin Cai, Jiayi Hou, Cindy Wen, Maryam Jafari, Xialin LiuLixia Luo, Jin Zhu, Austin Qiu, Rui Hou, Baoxin Chen, Jiangna Chen, David Granet, Christopher Heichel, Fu Shang, Xuri Li, Michal Krawczyk, Dorota Skowronska-Krawczyk, Yujuan Wang, William Shi, Daniel Chen, Zheng Zhong, Sheng Zhong, Liangfang Zhang, Shaochen Chen, Sean J. Morrison, Richard L. Maas, Kang Zhang, Yizhi Liu

Research output: Contribution to journal › Article › peer-review

156 Scopus citations

Abstract

The repair and regeneration of tissues using endogenous stem cells represents an ultimate goal in regenerative medicine. To our knowledge, human lens regeneration has not yet been demonstrated. Currently, the only treatment for cataracts, the leading cause of blindness worldwide, is to extract the cataractous lens and implant an artificial intraocular lens. However, this procedure poses notable risks of complications. Here we isolate lens epithelial stem/progenitor cells (LECs) in mammals and show that Pax6 and Bmi1 are required for LEC renewal. We design a surgical method of cataract removal that preserves endogenous LECs and achieves functional lens regeneration in rabbits and macaques, as well as in human infants with cataracts. Our method differs conceptually from current practice, as it preserves endogenous LECs and their natural environment maximally, and regenerates lenses with visual function. Our approach demonstrates a novel treatment strategy for cataracts and provides a new paradigm for tissue regeneration using endogenous stem cells.

Original language	English (US)
Pages (from-to)	323-328
Number of pages	6
Journal	Nature
Volume	531
Issue number	7594
DOIs	https://doi.org/10.1038/nature17181
State	Published - Mar 17 2016

ASJC Scopus subject areas

General

Access to Document

10.1038/nature17181

Cite this

Lin, H, Ouyang, H, Zhu, J, Huang, S, Liu, Z, Chen, S, Cao, G, Li, G, Signer, RAJ, Xu, Y, Chung, C, Zhang, Y, Lin, D, Patel, S, Wu, F, Cai, H, Hou, J, Wen, C, Jafari, M, Liu, X, Luo, L, Zhu, J, Qiu, A, Hou, R, Chen, B, Chen, J, Granet, D, Heichel, C, Shang, F, Li, X, Krawczyk, M, Skowronska-Krawczyk, D, Wang, Y, Shi, W, Chen, D, Zhong, Z, Zhong, S, Zhang, L, Chen, S, Morrison, SJ, Maas, RL, Zhang, K & Liu, Y 2016, 'Lens regeneration using endogenous stem cells with gain of visual function', Nature, vol. 531, no. 7594, pp. 323-328. https://doi.org/10.1038/nature17181

@article{bfc15e26188b4cfbad12dcce7e885bdc,

title = "Lens regeneration using endogenous stem cells with gain of visual function",

abstract = "The repair and regeneration of tissues using endogenous stem cells represents an ultimate goal in regenerative medicine. To our knowledge, human lens regeneration has not yet been demonstrated. Currently, the only treatment for cataracts, the leading cause of blindness worldwide, is to extract the cataractous lens and implant an artificial intraocular lens. However, this procedure poses notable risks of complications. Here we isolate lens epithelial stem/progenitor cells (LECs) in mammals and show that Pax6 and Bmi1 are required for LEC renewal. We design a surgical method of cataract removal that preserves endogenous LECs and achieves functional lens regeneration in rabbits and macaques, as well as in human infants with cataracts. Our method differs conceptually from current practice, as it preserves endogenous LECs and their natural environment maximally, and regenerates lenses with visual function. Our approach demonstrates a novel treatment strategy for cataracts and provides a new paradigm for tissue regeneration using endogenous stem cells.",

author = "Haotian Lin and Hong Ouyang and Jie Zhu and Shan Huang and Zhenzhen Liu and Shuyi Chen and Guiqun Cao and Gen Li and Signer, {Robert A J} and Yanxin Xu and Christopher Chung and Ying Zhang and Danni Lin and Sherrina Patel and Frances Wu and Huimin Cai and Jiayi Hou and Cindy Wen and Maryam Jafari and Xialin Liu and Lixia Luo and Jin Zhu and Austin Qiu and Rui Hou and Baoxin Chen and Jiangna Chen and David Granet and Christopher Heichel and Fu Shang and Xuri Li and Michal Krawczyk and Dorota Skowronska-Krawczyk and Yujuan Wang and William Shi and Daniel Chen and Zheng Zhong and Sheng Zhong and Liangfang Zhang and Shaochen Chen and Morrison, {Sean J.} and Maas, {Richard L.} and Kang Zhang and Yizhi Liu",

note = "Publisher Copyright: {\textcopyright} 2016 Macmillan Publishers Limited.",

year = "2016",

month = mar,

day = "17",

doi = "10.1038/nature17181",

language = "English (US)",

volume = "531",

pages = "323--328",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

number = "7594",

}

TY - JOUR

T1 - Lens regeneration using endogenous stem cells with gain of visual function

AU - Lin, Haotian

AU - Ouyang, Hong

AU - Zhu, Jie

AU - Huang, Shan

AU - Liu, Zhenzhen

AU - Chen, Shuyi

AU - Cao, Guiqun

AU - Li, Gen

AU - Signer, Robert A J

AU - Xu, Yanxin

AU - Chung, Christopher

AU - Zhang, Ying

AU - Lin, Danni

AU - Patel, Sherrina

AU - Wu, Frances

AU - Cai, Huimin

AU - Hou, Jiayi

AU - Wen, Cindy

AU - Jafari, Maryam

AU - Liu, Xialin

AU - Luo, Lixia

AU - Zhu, Jin

AU - Qiu, Austin

AU - Hou, Rui

AU - Chen, Baoxin

AU - Chen, Jiangna

AU - Granet, David

AU - Heichel, Christopher

AU - Shang, Fu

AU - Li, Xuri

AU - Krawczyk, Michal

AU - Skowronska-Krawczyk, Dorota

AU - Wang, Yujuan

AU - Shi, William

AU - Chen, Daniel

AU - Zhong, Zheng

AU - Zhong, Sheng

AU - Zhang, Liangfang

AU - Chen, Shaochen

AU - Morrison, Sean J.

AU - Maas, Richard L.

AU - Zhang, Kang

AU - Liu, Yizhi

PY - 2016/3/17

Y1 - 2016/3/17

N2 - The repair and regeneration of tissues using endogenous stem cells represents an ultimate goal in regenerative medicine. To our knowledge, human lens regeneration has not yet been demonstrated. Currently, the only treatment for cataracts, the leading cause of blindness worldwide, is to extract the cataractous lens and implant an artificial intraocular lens. However, this procedure poses notable risks of complications. Here we isolate lens epithelial stem/progenitor cells (LECs) in mammals and show that Pax6 and Bmi1 are required for LEC renewal. We design a surgical method of cataract removal that preserves endogenous LECs and achieves functional lens regeneration in rabbits and macaques, as well as in human infants with cataracts. Our method differs conceptually from current practice, as it preserves endogenous LECs and their natural environment maximally, and regenerates lenses with visual function. Our approach demonstrates a novel treatment strategy for cataracts and provides a new paradigm for tissue regeneration using endogenous stem cells.

AB - The repair and regeneration of tissues using endogenous stem cells represents an ultimate goal in regenerative medicine. To our knowledge, human lens regeneration has not yet been demonstrated. Currently, the only treatment for cataracts, the leading cause of blindness worldwide, is to extract the cataractous lens and implant an artificial intraocular lens. However, this procedure poses notable risks of complications. Here we isolate lens epithelial stem/progenitor cells (LECs) in mammals and show that Pax6 and Bmi1 are required for LEC renewal. We design a surgical method of cataract removal that preserves endogenous LECs and achieves functional lens regeneration in rabbits and macaques, as well as in human infants with cataracts. Our method differs conceptually from current practice, as it preserves endogenous LECs and their natural environment maximally, and regenerates lenses with visual function. Our approach demonstrates a novel treatment strategy for cataracts and provides a new paradigm for tissue regeneration using endogenous stem cells.

UR - http://www.scopus.com/inward/record.url?scp=84982187992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982187992&partnerID=8YFLogxK

U2 - 10.1038/nature17181

DO - 10.1038/nature17181

M3 - Article

C2 - 26958831

AN - SCOPUS:84982187992

SN - 0028-0836

VL - 531

SP - 323

EP - 328

JO - Nature

JF - Nature

IS - 7594

ER -

Lens regeneration using endogenous stem cells with gain of visual function

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this