Lentiviral transduction of murine oligodendrocytes in vivo

Sally R. McIver, Chul Sang Lee, Jin Moo Lee, Steven H. Green, Mark S. Sands, B. Joy Snider, Mark P. Goldberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV) or myelin basic protein (MBP), and two reporter sequences, cytosolic enhanced green fluorescent protein (eGFP) or a plasma membrane-targeted eGFP (human lymphocyte-specific protein tyrosine kinase [Lck]-eGFP). For all constructs, intracerebral injections to lateral corpus callosum resulted in wide-spread GFP expression in forebrain white matter glial cells. Intense cellular GFP fluorescence was observed within 3 days after injection and lasted for at least 28 days. The CMV promoter directed GFP expression in multiple glial cell types, whereas the MBP promoter targeted GFP specifically to oligodendrocytes. Expression of the membrane-targeted Lck-eGFP construct distinctly labeled individual myelinating processes of oligodendrocytes. Lentiviral constructs expressing eGFP or Lck-eGFP under the MBP promoter provide excellent visualization of oligodendrocyte morphology in intact white matter, and may prove valuable for delivering additional genes of interest to oligodendrocytes in vivo.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalJournal of Neuroscience Research
Volume82
Issue number3
DOIs
StatePublished - Nov 1 2005

Fingerprint

Oligodendroglia
Myelin Basic Protein
Cytomegalovirus
Neuroglia
Lentivirus
Vesicular Stomatitis
Injections
Corpus Callosum
Microinjections
Prosencephalon
Protein-Tyrosine Kinases
Genes
enhanced green fluorescent protein
Glycoproteins
Fluorescence
Cell Membrane
Lymphocytes
Viruses
Membranes
White Matter

Keywords

  • Lentiviral vectors
  • Morphology
  • Myelin
  • Oligodendrocyte
  • Transduction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

McIver, S. R., Lee, C. S., Lee, J. M., Green, S. H., Sands, M. S., Snider, B. J., & Goldberg, M. P. (2005). Lentiviral transduction of murine oligodendrocytes in vivo. Journal of Neuroscience Research, 82(3), 397-403. https://doi.org/10.1002/jnr.20626

Lentiviral transduction of murine oligodendrocytes in vivo. / McIver, Sally R.; Lee, Chul Sang; Lee, Jin Moo; Green, Steven H.; Sands, Mark S.; Snider, B. Joy; Goldberg, Mark P.

In: Journal of Neuroscience Research, Vol. 82, No. 3, 01.11.2005, p. 397-403.

Research output: Contribution to journalArticle

McIver, SR, Lee, CS, Lee, JM, Green, SH, Sands, MS, Snider, BJ & Goldberg, MP 2005, 'Lentiviral transduction of murine oligodendrocytes in vivo', Journal of Neuroscience Research, vol. 82, no. 3, pp. 397-403. https://doi.org/10.1002/jnr.20626
McIver SR, Lee CS, Lee JM, Green SH, Sands MS, Snider BJ et al. Lentiviral transduction of murine oligodendrocytes in vivo. Journal of Neuroscience Research. 2005 Nov 1;82(3):397-403. https://doi.org/10.1002/jnr.20626
McIver, Sally R. ; Lee, Chul Sang ; Lee, Jin Moo ; Green, Steven H. ; Sands, Mark S. ; Snider, B. Joy ; Goldberg, Mark P. / Lentiviral transduction of murine oligodendrocytes in vivo. In: Journal of Neuroscience Research. 2005 ; Vol. 82, No. 3. pp. 397-403.
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