Lentiviral transduction of murine oligodendrocytes in vivo

Sally R. McIver, Chul Sang Lee, Jin Moo Lee, Steven H. Green, Mark S. Sands, B. Joy Snider, Mark P. Goldberg

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV) or myelin basic protein (MBP), and two reporter sequences, cytosolic enhanced green fluorescent protein (eGFP) or a plasma membrane-targeted eGFP (human lymphocyte-specific protein tyrosine kinase [Lck]-eGFP). For all constructs, intracerebral injections to lateral corpus callosum resulted in wide-spread GFP expression in forebrain white matter glial cells. Intense cellular GFP fluorescence was observed within 3 days after injection and lasted for at least 28 days. The CMV promoter directed GFP expression in multiple glial cell types, whereas the MBP promoter targeted GFP specifically to oligodendrocytes. Expression of the membrane-targeted Lck-eGFP construct distinctly labeled individual myelinating processes of oligodendrocytes. Lentiviral constructs expressing eGFP or Lck-eGFP under the MBP promoter provide excellent visualization of oligodendrocyte morphology in intact white matter, and may prove valuable for delivering additional genes of interest to oligodendrocytes in vivo.

Original languageEnglish (US)
Pages (from-to)397-403
Number of pages7
JournalJournal of Neuroscience Research
Volume82
Issue number3
DOIs
StatePublished - Nov 1 2005

Keywords

  • Lentiviral vectors
  • Morphology
  • Myelin
  • Oligodendrocyte
  • Transduction

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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  • Cite this

    McIver, S. R., Lee, C. S., Lee, J. M., Green, S. H., Sands, M. S., Snider, B. J., & Goldberg, M. P. (2005). Lentiviral transduction of murine oligodendrocytes in vivo. Journal of Neuroscience Research, 82(3), 397-403. https://doi.org/10.1002/jnr.20626