Leydig insulin-like hormone, gubernacular development and testicular descent

Y. Kubota, S. Nef, P. J. Farmer, C. Temelcos, L. F. Parada, J. M. Hutson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Purpose: Testicular descent is controlled by 2 morphological and hormonal steps. Transabdominal testicular descent is mediated by gubernacular swelling and regression of the cranial suspensory ligament. Müllerian inhibiting substance (MIS) has been proposed to stimulate the swelling but this remains controversial. Recently, a mouse mutant for Leydig insulin-like hormone (Insl3) was found to have undescended testis and deficient gubernaculum. We examine the testicular position of Insl3 mutant mice and the development of gubernacula. Materials and Methods: Mice with Insl3 homozygotes (-/-), heterozygotes (+/-) and wildtypes (+/+) were examined at embryonic day 16.5 and birth. Macroscopic dissections and measurements of the testicular position, as well as microscopic analysis (hematoxylin and eosin, and Masson's trichrome) were performed. Results: Of the mice 11 Insl3 homozygote males had significantly impaired testicular descent at embryonic day 16.5 and birth (p <0.01), and the cord was thin and elongated, while 14 heterozygotes and 7 wild-types had normal testicular descent. Microscopically, the gubernaculum of Insl3 homozygotes was small with some muscle development but no central core of mesenchyme at embryonic day 16.5. On the other hand, heterozygotes and wild-types had normal gubernacular development with a swelling reaction. Conclusions: Insl3 mutants show feminized gubernaculum with deficient mesenchymal core. Insl3 appears to have some role in the gubernacular swelling reaction in mice.

Original languageEnglish (US)
Pages (from-to)1673-1675
Number of pages3
JournalJournal of Urology
Volume165
Issue number5 I
StatePublished - 2001

Fingerprint

Hormones
Insulin
Homozygote
Heterozygote
Parturition
Cryptorchidism
Muscle Development
Mesoderm
Hematoxylin
Eosine Yellowish-(YS)
Ligaments
Dissection
Gubernaculum

Keywords

  • Cryptorchidism
  • Leydig cells
  • Mullerian ducts
  • Testis

ASJC Scopus subject areas

  • Urology

Cite this

Kubota, Y., Nef, S., Farmer, P. J., Temelcos, C., Parada, L. F., & Hutson, J. M. (2001). Leydig insulin-like hormone, gubernacular development and testicular descent. Journal of Urology, 165(5 I), 1673-1675.

Leydig insulin-like hormone, gubernacular development and testicular descent. / Kubota, Y.; Nef, S.; Farmer, P. J.; Temelcos, C.; Parada, L. F.; Hutson, J. M.

In: Journal of Urology, Vol. 165, No. 5 I, 2001, p. 1673-1675.

Research output: Contribution to journalArticle

Kubota, Y, Nef, S, Farmer, PJ, Temelcos, C, Parada, LF & Hutson, JM 2001, 'Leydig insulin-like hormone, gubernacular development and testicular descent', Journal of Urology, vol. 165, no. 5 I, pp. 1673-1675.
Kubota Y, Nef S, Farmer PJ, Temelcos C, Parada LF, Hutson JM. Leydig insulin-like hormone, gubernacular development and testicular descent. Journal of Urology. 2001;165(5 I):1673-1675.
Kubota, Y. ; Nef, S. ; Farmer, P. J. ; Temelcos, C. ; Parada, L. F. ; Hutson, J. M. / Leydig insulin-like hormone, gubernacular development and testicular descent. In: Journal of Urology. 2001 ; Vol. 165, No. 5 I. pp. 1673-1675.
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