Abstract
Purpose: Testicular descent is controlled by 2 morphological and hormonal steps. Transabdominal testicular descent is mediated by gubernacular swelling and regression of the cranial suspensory ligament. Müllerian inhibiting substance (MIS) has been proposed to stimulate the swelling but this remains controversial. Recently, a mouse mutant for Leydig insulin-like hormone (Insl3) was found to have undescended testis and deficient gubernaculum. We examine the testicular position of Insl3 mutant mice and the development of gubernacula. Materials and Methods: Mice with Insl3 homozygotes (-/-), heterozygotes (+/-) and wildtypes (+/+) were examined at embryonic day 16.5 and birth. Macroscopic dissections and measurements of the testicular position, as well as microscopic analysis (hematoxylin and eosin, and Masson's trichrome) were performed. Results: Of the mice 11 Insl3 homozygote males had significantly impaired testicular descent at embryonic day 16.5 and birth (p <0.01), and the cord was thin and elongated, while 14 heterozygotes and 7 wild-types had normal testicular descent. Microscopically, the gubernaculum of Insl3 homozygotes was small with some muscle development but no central core of mesenchyme at embryonic day 16.5. On the other hand, heterozygotes and wild-types had normal gubernacular development with a swelling reaction. Conclusions: Insl3 mutants show feminized gubernaculum with deficient mesenchymal core. Insl3 appears to have some role in the gubernacular swelling reaction in mice.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1673-1675 |
| Number of pages | 3 |
| Journal | Journal of Urology |
| Volume | 165 |
| Issue number | 5 I |
| State | Published - 2001 |
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Keywords
- Cryptorchidism
- Leydig cells
- Mullerian ducts
- Testis
ASJC Scopus subject areas
- Urology
Cite this
Leydig insulin-like hormone, gubernacular development and testicular descent. / Kubota, Y.; Nef, S.; Farmer, P. J.; Temelcos, C.; Parada, L. F.; Hutson, J. M.
In: Journal of Urology, Vol. 165, No. 5 I, 2001, p. 1673-1675.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Leydig insulin-like hormone, gubernacular development and testicular descent
AU - Kubota, Y.
AU - Nef, S.
AU - Farmer, P. J.
AU - Temelcos, C.
AU - Parada, L. F.
AU - Hutson, J. M.
PY - 2001
Y1 - 2001
N2 - Purpose: Testicular descent is controlled by 2 morphological and hormonal steps. Transabdominal testicular descent is mediated by gubernacular swelling and regression of the cranial suspensory ligament. Müllerian inhibiting substance (MIS) has been proposed to stimulate the swelling but this remains controversial. Recently, a mouse mutant for Leydig insulin-like hormone (Insl3) was found to have undescended testis and deficient gubernaculum. We examine the testicular position of Insl3 mutant mice and the development of gubernacula. Materials and Methods: Mice with Insl3 homozygotes (-/-), heterozygotes (+/-) and wildtypes (+/+) were examined at embryonic day 16.5 and birth. Macroscopic dissections and measurements of the testicular position, as well as microscopic analysis (hematoxylin and eosin, and Masson's trichrome) were performed. Results: Of the mice 11 Insl3 homozygote males had significantly impaired testicular descent at embryonic day 16.5 and birth (p <0.01), and the cord was thin and elongated, while 14 heterozygotes and 7 wild-types had normal testicular descent. Microscopically, the gubernaculum of Insl3 homozygotes was small with some muscle development but no central core of mesenchyme at embryonic day 16.5. On the other hand, heterozygotes and wild-types had normal gubernacular development with a swelling reaction. Conclusions: Insl3 mutants show feminized gubernaculum with deficient mesenchymal core. Insl3 appears to have some role in the gubernacular swelling reaction in mice.
AB - Purpose: Testicular descent is controlled by 2 morphological and hormonal steps. Transabdominal testicular descent is mediated by gubernacular swelling and regression of the cranial suspensory ligament. Müllerian inhibiting substance (MIS) has been proposed to stimulate the swelling but this remains controversial. Recently, a mouse mutant for Leydig insulin-like hormone (Insl3) was found to have undescended testis and deficient gubernaculum. We examine the testicular position of Insl3 mutant mice and the development of gubernacula. Materials and Methods: Mice with Insl3 homozygotes (-/-), heterozygotes (+/-) and wildtypes (+/+) were examined at embryonic day 16.5 and birth. Macroscopic dissections and measurements of the testicular position, as well as microscopic analysis (hematoxylin and eosin, and Masson's trichrome) were performed. Results: Of the mice 11 Insl3 homozygote males had significantly impaired testicular descent at embryonic day 16.5 and birth (p <0.01), and the cord was thin and elongated, while 14 heterozygotes and 7 wild-types had normal testicular descent. Microscopically, the gubernaculum of Insl3 homozygotes was small with some muscle development but no central core of mesenchyme at embryonic day 16.5. On the other hand, heterozygotes and wild-types had normal gubernacular development with a swelling reaction. Conclusions: Insl3 mutants show feminized gubernaculum with deficient mesenchymal core. Insl3 appears to have some role in the gubernacular swelling reaction in mice.
KW - Cryptorchidism
KW - Leydig cells
KW - Mullerian ducts
KW - Testis
UR - http://www.scopus.com/inward/record.url?scp=0035043428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035043428&partnerID=8YFLogxK
M3 - Article
C2 - 11342953
AN - SCOPUS:0035043428
VL - 165
SP - 1673
EP - 1675
JO - Journal of Urology
JF - Journal of Urology
SN - 0022-5347
IS - 5 I
ER -